Detailed information NTG0974734

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QDT75_RS13960
• Genome accession: NZ_CP151078
• Coordinates: 2956964..2958082 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain PUMA0052
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2951964..2963082

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QDT75_RS13950 (QDT75_13950)	-	2955088..2955780 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
QDT75_RS13955 (QDT75_13955)	pilT	2955907..2956944 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
QDT75_RS13960 (QDT75_13960)	pilU	2956964..2958082 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QDT75_RS13965 (QDT75_13965)	fur	2958152..2958589 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
QDT75_RS13970 (QDT75_13970)	bamE	2958701..2959099 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
QDT75_RS13975 (QDT75_13975)	-	2959154..2959477 (-)	324	WP_000442589.1	RnfH family protein	-
QDT75_RS13980 (QDT75_13980)	-	2959477..2960544 (-)	1068	WP_272317396.1	hypothetical protein	-
QDT75_RS13985 (QDT75_13985)	-	2960697..2961152 (-)	456	WP_000782976.1	bacteriohemerythrin	-
QDT75_RS13990 (QDT75_13990)	-	2961405..2962253 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=974734 QDT75_RS13960 WP_000347034.1 2956964..2958082(+) (pilU) [Acinetobacter baumannii strain PUMA0052]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=974734 QDT75_RS13960 WP_000347034.1 2956964..2958082(+) (pilU) [Acinetobacter baumannii strain PUMA0052]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCACCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39