Detailed information NTG0974372

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACK8DD_RS14090
• Genome accession: NZ_CP179880
• Coordinates: 2996580..2997698 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347040.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain MRSN548254
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2991580..3002698

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK8DD_RS14080 (ACK8DD_14080)	-	2994696..2995388 (-)	693	WP_004748094.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACK8DD_RS14085 (ACK8DD_14085)	pilT	2995515..2996552 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACK8DD_RS14090 (ACK8DD_14090)	pilU	2996580..2997698 (+)	1119	WP_000347040.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACK8DD_RS14095 (ACK8DD_14095)	fur	2997768..2998205 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACK8DD_RS14100 (ACK8DD_14100)	-	2998317..2998715 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACK8DD_RS14105 (ACK8DD_14105)	-	2998770..2999093 (-)	324	WP_000442590.1	RnfH family protein	-
ACK8DD_RS14110 (ACK8DD_14110)	-	2999093..3000160 (-)	1068	WP_004748097.1	hypothetical protein	-
ACK8DD_RS14115 (ACK8DD_14115)	-	3000313..3000768 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ACK8DD_RS14120 (ACK8DD_14120)	-	3001021..3001869 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41896.10 Da
• Isoelectric Point: 6.6406

>NTDB_id=974372 ACK8DD_RS14090 WP_000347040.1 2996580..2997698(+) (pilU) [Acinetobacter baumannii strain MRSN548254]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=974372 ACK8DD_RS14090 WP_000347040.1 2996580..2997698(+) (pilU) [Acinetobacter baumannii strain MRSN548254]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392