Detailed information NTG0974113

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACK8DA_RS04360
• Genome accession: NZ_CP179869
• Coordinates: 919027..920145 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347040.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain MRSN122172
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 914027..925145

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK8DA_RS04330 (ACK8DA_04330)	-	914856..915704 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
ACK8DA_RS04335 (ACK8DA_04335)	-	915957..916412 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ACK8DA_RS04340 (ACK8DA_04340)	-	916565..917632 (+)	1068	WP_046693192.1	membrane protein	-
ACK8DA_RS04345 (ACK8DA_04345)	-	917632..917955 (+)	324	WP_000442590.1	RnfH family protein	-
ACK8DA_RS04350 (ACK8DA_04350)	-	918010..918408 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACK8DA_RS04355 (ACK8DA_04355)	fur	918520..918957 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACK8DA_RS04360 (ACK8DA_04360)	pilU	919027..920145 (-)	1119	WP_000347040.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACK8DA_RS04365 (ACK8DA_04365)	pilT	920173..921210 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACK8DA_RS04370 (ACK8DA_04370)	-	921337..922029 (+)	693	WP_004748094.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41896.10 Da
• Isoelectric Point: 6.6406

>NTDB_id=974113 ACK8DA_RS04360 WP_000347040.1 919027..920145(-) (pilU) [Acinetobacter baumannii strain MRSN122172]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=974113 ACK8DA_RS04360 WP_000347040.1 919027..920145(-) (pilU) [Acinetobacter baumannii strain MRSN122172]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392