Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   WOB24_RS15100 Genome accession   NZ_CP150891
Coordinates   3207140..3208477 (+) Length   445 a.a.
NCBI ID   WP_339080118.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP25     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3202140..3213477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOB24_RS15075 (WOB24_15075) pgeF 3202210..3202935 (-) 726 WP_341303797.1 peptidoglycan editing factor PgeF -
  WOB24_RS15080 (WOB24_15080) rluD 3202968..3203942 (-) 975 WP_341303798.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  WOB24_RS15085 (WOB24_15085) - 3204090..3205073 (+) 984 WP_339080115.1 outer membrane protein assembly factor BamD -
  WOB24_RS15090 (WOB24_15090) - 3205313..3205558 (+) 246 WP_339080116.1 PP0621 family protein -
  WOB24_RS15095 (WOB24_15095) pilS 3205548..3207137 (+) 1590 WP_341303799.1 ATP-binding protein Regulator
  WOB24_RS15100 (WOB24_15100) pilR 3207140..3208477 (+) 1338 WP_339080118.1 sigma-54 dependent transcriptional regulator Regulator
  WOB24_RS15105 (WOB24_15105) thiO 3208645..3209748 (-) 1104 WP_341303800.1 glycine oxidase ThiO -
  WOB24_RS15110 (WOB24_15110) - 3209817..3210332 (+) 516 WP_341303801.1 GspH/FimT family pseudopilin -
  WOB24_RS15115 (WOB24_15115) - 3210431..3210913 (+) 483 WP_341303802.1 GspH/FimT family pseudopilin -
  WOB24_RS15120 (WOB24_15120) pilV 3210907..3211422 (+) 516 WP_341303803.1 type IV pilus modification protein PilV -
  WOB24_RS15125 (WOB24_15125) - 3211419..3212057 (+) 639 WP_341303804.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WOB24_RS15130 (WOB24_15130) - 3212084..3212464 (+) 381 WP_341303805.1 pilus assembly PilX N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49807.24 Da        Isoelectric Point: 5.6283

>NTDB_id=973534 WOB24_RS15100 WP_339080118.1 3207140..3208477(+) (pilR) [Pseudomonas sp. TMP25]
MTRQRALIVDDEPDIRELLEITLGRMKIDTRSARNVKEAREWLAKEPFDLCLTDMRLPDGTGLELVQHILQRHPQVPVAM
ITAYGSLDTAINALKAGAFDFLTKPVDLTRLRELVAAALRLRSADGEELPVDSRLLGESPPMKTLRKQILKLARSQAPVY
ISGESGSGKELVARLIHEQGSRGEQPFVPVNCGAIPTELMESEFFGHKKGSFTGAIEDKQGLFQAANGGTLFLDEVADLP
LPMQVKLLRAIQEKAVRTVGGQQEVIVDVRILCATHKDLAAEVAAERFRQDLYYRLNVIELRVPALRERREDIALLADSM
LKRLAEGTGISAAKLDEEALEKLKSYRFPGNVRELENMLERAYTLCEDDRISASDLRLADCSPTNESGETSLAQVDNLED
HLEAIERKLIMQALEETRWNRTAAAQRLGLSFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=973534 WOB24_RS15100 WP_339080118.1 3207140..3208477(+) (pilR) [Pseudomonas sp. TMP25]
ATGACCCGCCAGAGAGCCCTGATCGTCGATGACGAACCCGATATTCGCGAACTCCTGGAAATCACCCTAGGCCGAATGAA
AATCGACACCCGCAGCGCACGCAACGTCAAAGAAGCCCGCGAATGGCTGGCCAAAGAGCCGTTTGATCTGTGCCTGACCG
ACATGCGCCTGCCCGACGGCACTGGCCTGGAGTTGGTGCAGCACATCTTGCAACGCCATCCGCAGGTGCCGGTGGCAATG
ATCACCGCCTATGGCAGCCTCGACACCGCCATCAACGCCTTAAAAGCCGGCGCATTCGACTTCCTAACCAAGCCCGTCGA
CCTCACACGCTTGCGCGAACTTGTCGCCGCCGCTCTGCGCCTGCGCAGCGCCGATGGCGAAGAGCTGCCAGTGGACAGCC
GCCTGCTTGGCGAATCGCCACCGATGAAGACCTTGCGCAAACAAATCCTCAAACTGGCTCGCAGCCAAGCCCCGGTGTAT
ATCAGCGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCTCGCCTGATCCACGAACAAGGCTCGCGCGGCGAACAACCGTT
TGTGCCGGTCAACTGCGGCGCCATTCCGACCGAGTTGATGGAAAGTGAGTTTTTCGGCCACAAAAAAGGCAGCTTTACCG
GCGCCATCGAAGACAAACAAGGCCTGTTCCAGGCAGCCAATGGCGGCACGCTGTTCCTTGATGAGGTCGCCGATCTGCCA
CTGCCGATGCAGGTCAAACTGCTACGCGCCATCCAGGAAAAAGCCGTGCGCACGGTGGGCGGCCAACAGGAAGTCATCGT
CGATGTGCGCATCCTCTGCGCCACCCATAAAGACTTGGCCGCAGAAGTCGCCGCTGAGCGCTTCCGCCAAGACCTTTACT
ACCGCCTTAACGTCATTGAACTGCGCGTGCCTGCCCTGCGCGAGCGCCGTGAAGACATCGCCCTACTCGCCGACAGCATG
CTCAAGCGCCTGGCCGAAGGCACCGGCATAAGCGCCGCCAAGCTGGATGAAGAGGCACTGGAAAAGCTCAAGAGCTACCG
CTTCCCCGGCAACGTGCGCGAGCTGGAGAACATGCTCGAGCGTGCCTATACCCTGTGTGAAGACGACCGCATCAGCGCCA
GCGACCTCCGCTTAGCCGATTGCAGCCCCACCAATGAAAGCGGCGAAACCAGCCTCGCACAGGTCGACAATTTAGAAGAC
CACCTAGAAGCCATCGAGCGTAAATTGATCATGCAGGCGCTGGAGGAAACCCGCTGGAACCGCACCGCCGCCGCGCAACG
CCTAGGCCTAAGCTTCCGCTCGATGCGCTATCGCCTGAAAAAACTCGGCATTGATTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

84.719

100

0.847
  pilR Acinetobacter baumannii strain A118

49.891

100

0.515
  luxO Vibrio cholerae strain A1552

38.789

100

0.389

Multiple sequence alignment