Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   WOB24_RS15095 Genome accession   NZ_CP150891
Coordinates   3205548..3207137 (+) Length   529 a.a.
NCBI ID   WP_341303799.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP25     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3200548..3212137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOB24_RS15075 (WOB24_15075) pgeF 3202210..3202935 (-) 726 WP_341303797.1 peptidoglycan editing factor PgeF -
  WOB24_RS15080 (WOB24_15080) rluD 3202968..3203942 (-) 975 WP_341303798.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  WOB24_RS15085 (WOB24_15085) - 3204090..3205073 (+) 984 WP_339080115.1 outer membrane protein assembly factor BamD -
  WOB24_RS15090 (WOB24_15090) - 3205313..3205558 (+) 246 WP_339080116.1 PP0621 family protein -
  WOB24_RS15095 (WOB24_15095) pilS 3205548..3207137 (+) 1590 WP_341303799.1 ATP-binding protein Regulator
  WOB24_RS15100 (WOB24_15100) pilR 3207140..3208477 (+) 1338 WP_339080118.1 sigma-54 dependent transcriptional regulator Regulator
  WOB24_RS15105 (WOB24_15105) thiO 3208645..3209748 (-) 1104 WP_341303800.1 glycine oxidase ThiO -
  WOB24_RS15110 (WOB24_15110) - 3209817..3210332 (+) 516 WP_341303801.1 GspH/FimT family pseudopilin -
  WOB24_RS15115 (WOB24_15115) - 3210431..3210913 (+) 483 WP_341303802.1 GspH/FimT family pseudopilin -
  WOB24_RS15120 (WOB24_15120) pilV 3210907..3211422 (+) 516 WP_341303803.1 type IV pilus modification protein PilV -
  WOB24_RS15125 (WOB24_15125) - 3211419..3212057 (+) 639 WP_341303804.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58790.07 Da        Isoelectric Point: 8.9744

>NTDB_id=973533 WOB24_RS15095 WP_341303799.1 3205548..3207137(+) (pilS) [Pseudomonas sp. TMP25]
MGTEFALSGEQSWRVLRLYHLYRLIIGITLVLLVSSNLDTELLEMAHPTLFRNGSWLYLVLNVLVIVTLRQPKRLAQVFS
LALVDVVLLSGLFYAAGGTPSGIGNLLIVAVAIANILLRGRIGLLIAAVAAIGMIYLTFYLSLSRPGASAQYVQVGAFGA
LCFAAALFVQGLTRRVQTSERLAEQRAADVANLEALNALILQRMRTGILVLDEVHRVLLANQGANHLLGHADLTGKILDP
HCPQLIKRMQQWLYNPSLRPASLQAEPDGRVLQPSFIALQRGERKHTLIFLDDISQIAQQAQQLKLASLGRLTAGIAHEI
RNPLGAISHAAQLLQESEELAGADLRLAQIIQDHSQRMNMVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRTRAAPNH
TLHLETSSGSIQTRMDPHQLTQVLSNLVENGLRYSAQKNKQGQVWLKLFRDPDSELPVLEVLDDGPGIADDQLHHLFEPF
YTTETKGTGLGLYLSRELCESNQARLDYKVRESGGSCLRITFAHPRKLS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=973533 WOB24_RS15095 WP_341303799.1 3205548..3207137(+) (pilS) [Pseudomonas sp. TMP25]
GTGGGGACTGAGTTCGCCCTCAGCGGGGAACAAAGCTGGCGGGTTTTGCGGCTCTACCACCTGTACCGGCTGATTATCGG
CATTACCTTGGTGTTGTTGGTATCCAGCAACCTCGACACCGAATTATTGGAAATGGCCCACCCGACGCTGTTTCGTAACG
GCAGTTGGTTGTACCTGGTGCTCAACGTTTTAGTCATCGTCACCCTGCGTCAACCCAAACGTCTGGCGCAGGTGTTTAGC
TTGGCGTTGGTGGATGTGGTGCTGCTCTCCGGCCTGTTCTACGCCGCCGGCGGCACGCCCAGCGGCATCGGCAACTTGTT
AATCGTGGCCGTGGCGATCGCCAATATTCTCTTGCGTGGCCGCATCGGCTTGCTGATTGCAGCCGTTGCCGCCATTGGCA
TGATCTACCTGACCTTCTACCTCAGCCTCAGCCGGCCAGGCGCTTCCGCCCAGTACGTGCAAGTGGGTGCATTTGGCGCG
CTGTGTTTTGCGGCAGCCCTGTTTGTGCAGGGCCTGACCCGCCGCGTGCAAACCAGCGAACGCTTGGCCGAGCAGCGTGC
CGCCGATGTCGCCAACCTAGAAGCGTTGAACGCGTTGATATTGCAGCGCATGCGCACCGGCATTCTGGTGCTGGATGAAG
TGCATCGCGTGCTGCTGGCCAACCAAGGCGCCAACCACCTACTTGGGCATGCCGACCTAACCGGGAAAATCCTTGACCCG
CATTGTCCGCAATTGATCAAACGCATGCAGCAGTGGCTATACAACCCAAGCCTGCGCCCTGCCAGCCTGCAAGCCGAACC
AGACGGGCGCGTGTTGCAGCCGAGCTTTATCGCCCTGCAGCGCGGCGAGCGCAAACACACGCTGATTTTCCTAGATGACA
TCTCGCAAATCGCCCAACAGGCGCAGCAACTCAAGCTCGCCTCATTAGGTCGCTTGACGGCCGGCATCGCCCACGAGATT
CGCAACCCGCTGGGCGCCATCAGTCACGCCGCGCAGCTGCTGCAAGAGTCGGAAGAATTAGCCGGGGCAGACCTGCGCTT
GGCGCAAATCATTCAGGATCACTCGCAGCGCATGAATATGGTGATCGAGAACGTCCTGCAACTGTCGCGGCGGCGCCAAG
CCGAGCCGCAATTGCTCGATTTAAAATACTGGCTGCACCGTTTTGCCAGCGAGTTCCGTACCCGCGCCGCGCCCAACCAT
ACACTGCACCTGGAAACCAGCAGCGGTAGCATCCAAACCCGTATGGACCCGCACCAGCTCACCCAAGTACTGAGCAACCT
CGTGGAAAATGGCCTGCGCTACAGCGCGCAAAAGAACAAACAGGGGCAGGTCTGGCTGAAACTGTTCCGCGATCCAGACA
GCGAATTACCGGTGCTTGAGGTGCTGGATGACGGCCCAGGGATTGCCGACGATCAACTGCATCACCTCTTTGAGCCCTTC
TACACCACTGAAACCAAAGGCACTGGCCTGGGCCTGTATTTGTCTCGTGAACTGTGCGAAAGCAACCAAGCCCGCCTCGA
CTATAAAGTGCGCGAAAGTGGCGGCAGCTGCCTGCGCATCACCTTCGCCCACCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(416-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(308-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

67.878

98.866

0.671

Multiple sequence alignment