Detailed information NTG0973524

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WOB24_RS08635
• Genome accession: NZ_CP150891
• Coordinates: 1797476..1798702 (+)
• Length: 408 a.a.
• NCBI ID: WP_341305278.1
• Uniprot ID: -
• Organism: Pseudomonas sp. TMP25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1792476..1803702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WOB24_RS08610 (WOB24_08610)	-	1793016..1793846 (+)	831	WP_339083084.1	undecaprenyl-diphosphate phosphatase	-
WOB24_RS08615 (WOB24_08615)	-	1793931..1794896 (+)	966	WP_341305274.1	alpha/beta hydrolase	-
WOB24_RS08620 (WOB24_08620)	-	1794970..1796556 (-)	1587	WP_341305275.1	ABC-F family ATPase	-
WOB24_RS08625 (WOB24_08625)	-	1796696..1797049 (-)	354	WP_341305276.1	MmcQ/YjbR family DNA-binding protein	-
WOB24_RS08630 (WOB24_08630)	-	1797055..1797333 (-)	279	WP_341305277.1	peptidylprolyl isomerase	-
WOB24_RS08635 (WOB24_08635)	pilU	1797476..1798702 (+)	1227	WP_341305278.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WOB24_RS08640 (WOB24_08640)	-	1798745..1800700 (-)	1956	WP_341305279.1	acetoacetate--CoA ligase	-
WOB24_RS08645 (WOB24_08645)	-	1801098..1802000 (-)	903	WP_341305280.1	hydroxymethylglutaryl-CoA lyase	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 44666.11 Da
• Isoelectric Point: 6.1330

>NTDB_id=973524 WOB24_RS08635 WP_341305278.1 1797476..1798702(+) (pilU) [Pseudomonas sp. TMP25]
MDLNAMLKVLSSQDGSDLYLSTGAPPCAKFNGVLKALSAEVLKAGEVAAIAESVMDSAQREEFERELEMNLAISIAGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTIRDLIKRGDFGDIKEIMEKSKNLGMQTFDQAL
IDLVNDGSIDEEEAVKNADSANNVRLKLKLYRDNPTASVPVEAAVPAPVAAAPRPPAPAASAGEWGLELKLEDIEHDLPP
EDPGRKGI

Nucleotide

• Length: 1227 bp

>NTDB_id=973524 WOB24_RS08635 WP_341305278.1 1797476..1798702(+) (pilU) [Pseudomonas sp. TMP25]
ATGGACCTCAATGCAATGCTCAAAGTCCTGTCCAGTCAGGACGGCTCCGACCTTTATCTGTCAACCGGTGCGCCGCCCTG
CGCTAAGTTCAACGGCGTGCTAAAGGCGCTGAGTGCCGAAGTGCTTAAGGCCGGCGAGGTGGCAGCAATCGCTGAGTCGG
TGATGGACAGTGCGCAGCGCGAAGAATTTGAGCGTGAGTTGGAGATGAACTTAGCGATTTCCATCGCCGGCGTTGGGCGT
TTCCGCATCAATATTTTTAAGCAGCGCAACGAAGTGTCGATTGTGGCGCGCAATATCAAGTTGGATATCCCCAAGTTCGA
AGACCTCAAACTGCCGGAGGTGTTGCTTAAAACCGTGATGGAAAAACGCGGCCTGGTGTTGTTTGTGGGCGGTACCGGCT
CAGGTAAGTCGACCTCGCTGGCGGCCTTAATCGATTACCGCAACCGCAACAGTGGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTATGTGCACAAGCACAAAAAGTCGATCATCAACCAGCGTGAAGTCGGGGTCGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCTTAATTGGCGAAATTCGTGACCGCGAAACCATGGAGCATG
CGCTGGCGTTTGCTGACACCGGCCACCTGGCGATTTCCACGCTGCACGCCAACAACGCCAATCAAGCGCTGGACCGCATC
ATCAATTTTTTTCCGGAAGATCGTCGCCCGCAGTTGCTCAACGACCTGGGCAACAATCTCAAGGCTTTTGTTTCGCAGCG
GCTGGTGAAAACCGTCGACGGCAAGCGCCGCGCGGCAGTCGAGGTGCTGCTGGGCACGCCAACCATTCGTGACTTGATCA
AACGCGGTGATTTTGGCGATATCAAAGAGATCATGGAGAAATCCAAGAACCTCGGCATGCAAACCTTCGATCAGGCACTC
ATCGACTTGGTCAATGACGGTTCCATTGATGAAGAAGAAGCCGTTAAGAACGCCGATTCGGCCAACAACGTGCGCCTCAA
GCTTAAGCTTTATCGGGACAATCCCACCGCATCGGTGCCGGTTGAAGCAGCAGTCCCTGCGCCAGTTGCAGCTGCGCCGC
GGCCGCCAGCGCCCGCTGCTTCGGCCGGTGAGTGGGGCCTTGAGTTGAAGCTGGAAGATATTGAGCATGACCTGCCGCCC
GAAGATCCCGGGCGTAAGGGCATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (47-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	86.029	0.483
pilU	Acinetobacter baylyi ADP1	52.557	86.275	0.453
pilU	Vibrio cholerae strain A1552	51.532	87.99	0.453
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.66	95.098	0.368