Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   WOC44_RS10300 Genome accession   NZ_CP150737
Coordinates   2019852..2020355 (-) Length   167 a.a.
NCBI ID   WP_000934799.1    Uniprot ID   A0A7U7IE99
Organism   Staphylococcus aureus strain TUM22723     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2018025..2050305 2019852..2020355 within 0


Gene organization within MGE regions


Location: 2018025..2050305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC44_RS10290 (WOC44_10290) - 2018354..2019292 (+) 939 WP_001822249.1 Abi family protein -
  WOC44_RS10295 (WOC44_10295) rpsR 2019558..2019800 (-) 243 WP_000897044.1 30S ribosomal protein S18 -
  WOC44_RS10300 (WOC44_10300) ssbA 2019852..2020355 (-) 504 WP_000934799.1 single-stranded DNA-binding protein Machinery gene
  WOC44_RS10305 (WOC44_10305) rpsF 2020376..2020672 (-) 297 WP_001261460.1 30S ribosomal protein S6 -
  WOC44_RS10310 (WOC44_10310) - 2020841..2021125 (+) 285 WP_001553897.1 hypothetical protein -
  WOC44_RS10315 (WOC44_10315) - 2021282..2021584 (+) 303 WP_001553895.1 hypothetical protein -
  WOC44_RS10320 (WOC44_10320) - 2021627..2021962 (+) 336 WP_001553893.1 DUF961 family protein -
  WOC44_RS10325 (WOC44_10325) - 2021966..2022268 (+) 303 WP_341270885.1 hypothetical protein -
  WOC44_RS10330 (WOC44_10330) - 2022458..2023525 (+) 1068 WP_341270886.1 replication initiation factor domain-containing protein -
  WOC44_RS10335 (WOC44_10335) - 2023611..2024672 (+) 1062 WP_031904062.1 conjugal transfer protein -
  WOC44_RS10340 (WOC44_10340) - 2024677..2024937 (+) 261 WP_001553885.1 TcpD family membrane protein -
  WOC44_RS10345 (WOC44_10345) - 2024943..2025332 (+) 390 WP_031904063.1 TcpE family conjugal transfer membrane protein -
  WOC44_RS10350 (WOC44_10350) - 2025367..2027862 (+) 2496 WP_001553882.1 ATP-binding protein -
  WOC44_RS10355 (WOC44_10355) - 2028095..2029453 (+) 1359 WP_001553878.1 FtsK/SpoIIIE domain-containing protein -
  WOC44_RS10360 (WOC44_10360) - 2029460..2029627 (+) 168 WP_001553877.1 hypothetical protein -
  WOC44_RS10365 (WOC44_10365) - 2029643..2031559 (+) 1917 WP_001553875.1 hypothetical protein -
  WOC44_RS10370 (WOC44_10370) - 2031549..2032586 (+) 1038 WP_001553872.1 CHAP domain-containing protein -
  WOC44_RS10375 (WOC44_10375) - 2032593..2033186 (+) 594 WP_031904065.1 hypothetical protein -
  WOC44_RS10380 (WOC44_10380) - 2033234..2033596 (+) 363 WP_031904066.1 cystatin-like fold lipoprotein -
  WOC44_RS10385 (WOC44_10385) - 2033708..2034238 (+) 531 WP_108957673.1 helix-turn-helix domain-containing protein -
  WOC44_RS10390 (WOC44_10390) - 2034267..2034692 (+) 426 WP_232051005.1 IS30 family transposase -
  WOC44_RS10395 (WOC44_10395) - 2035117..2035308 (+) 192 WP_001052483.1 hypothetical protein -
  WOC44_RS10400 (WOC44_10400) ychF 2035394..2036491 (-) 1098 WP_001218732.1 redox-regulated ATPase YchF -
  WOC44_RS10405 (WOC44_10405) - 2036503..2036706 (-) 204 WP_000157345.1 DUF951 domain-containing protein -
  WOC44_RS10410 (WOC44_10410) - 2036736..2037617 (-) 882 WP_001077602.1 mechanosensitive ion channel family protein -
  WOC44_RS10415 (WOC44_10415) - 2037774..2038619 (-) 846 WP_001550052.1 ParB/RepB/Spo0J family partition protein -
  WOC44_RS10420 (WOC44_10420) - 2039283..2040386 (+) 1104 Protein_2007 PLP-dependent transferase -
  WOC44_RS10425 (WOC44_10425) metC 2040383..2041543 (+) 1161 WP_000175846.1 cystathionine beta-lyase MetC -
  WOC44_RS10430 (WOC44_10430) - 2041512..2043353 (+) 1842 WP_000077318.1 bifunctional homocysteine S-methyltransferase/methylenetetrahydrofolate reductase -
  WOC44_RS10435 (WOC44_10435) metE 2043350..2045578 (+) 2229 WP_000207614.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  WOC44_RS10440 (WOC44_10440) - 2045621..2046373 (+) 753 WP_000195907.1 cyclase family protein -
  WOC44_RS10445 (WOC44_10445) - 2046456..2047637 (-) 1182 WP_000199070.1 acetyl-CoA C-acetyltransferase -
  WOC44_RS10450 (WOC44_10450) - 2048176..2049309 (+) 1134 WP_000245700.1 low temperature requirement protein A -
  WOC44_RS10455 (WOC44_10455) - 2049676..2050305 (-) 630 WP_000677714.1 ABC-2 transporter permease -

Sequence


Protein


Download         Length: 167 a.a.        Molecular weight: 18539.12 Da        Isoelectric Point: 4.7305

>NTDB_id=971496 WOC44_RS10300 WP_000934799.1 2019852..2020355(-) (ssbA) [Staphylococcus aureus strain TUM22723]
MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGEREADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSR
NYENQEGRRVFVTEVVCDSVQFLEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
SDDDLPF

Nucleotide


Download         Length: 504 bp        

>NTDB_id=971496 WOC44_RS10300 WP_000934799.1 2019852..2020355(-) (ssbA) [Staphylococcus aureus strain TUM22723]
ATGCTAAATAGAGTTGTATTAGTAGGTCGTTTAACGAAAGATCCCGAATACAGAACCACTCCCTCAGGTGTGAGTGTAGC
GACATTCACTCTTGCAGTAAATCGTACGTTCACGAATGCTCAAGGGGAGCGCGAAGCAGATTTTATTAACTGTGTTGTTT
TTAGAAGACAAGCAGATAATGTAAATAACTATTTATCTAAAGGTAGTTTAGCTGGTGTAGATGGTCGCTTACAATCCCGT
AATTATGAAAATCAAGAAGGTCGTCGTGTGTTTGTTACTGAAGTTGTGTGTGATAGCGTTCAATTCCTTGAACCTAAAAA
TGCGCAACAAAATGGTGGCCAACGTCAACAAAATGAATTCCAAGATTACGGTCAAGGATTCGGTGGTCAACAATCAGGAC
AAAACAATTCGTACAATAATTCATCAAACACGAAACAATCTGATAATCCATTTGCAAATGCAAACGGACCGATTGATATA
AGTGATGATGACTTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A7U7IE99

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

65.341

100

0.689

  ssb Latilactobacillus sakei subsp. sakei 23K

54.971

100

0.563

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

63.473

0.371

  ssb Glaesserella parasuis strain SC1401

34.463

100

0.365


Multiple sequence alignment