Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   WOC73_RS05155 Genome accession   NZ_CP150735
Coordinates   1058011..1058508 (-) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22730     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1053011..1063508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC73_RS05130 (WOC73_05130) gcvPB 1053173..1054645 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  WOC73_RS05135 (WOC73_05135) gcvPA 1054638..1055984 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  WOC73_RS05140 (WOC73_05140) gcvT 1056004..1057095 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  WOC73_RS05145 (WOC73_05145) - 1057254..1057778 (-) 525 WP_341261387.1 shikimate kinase -
  WOC73_RS05150 (WOC73_05150) - 1057768..1057914 (-) 147 WP_001789879.1 hypothetical protein -
  WOC73_RS05155 (WOC73_05155) comGF 1058011..1058508 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  WOC73_RS05160 (WOC73_05160) comGE 1058426..1058725 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  WOC73_RS05165 (WOC73_05165) comGD 1058712..1059158 (-) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  WOC73_RS05170 (WOC73_05170) comGC 1059136..1059447 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  WOC73_RS05175 (WOC73_05175) comGB 1059461..1060531 (-) 1071 WP_000776421.1 competence type IV pilus assembly protein ComGB Machinery gene
  WOC73_RS05180 (WOC73_05180) comGA 1060503..1061477 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  WOC73_RS05185 (WOC73_05185) - 1061529..1062152 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  WOC73_RS05190 (WOC73_05190) - 1062149..1062478 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  WOC73_RS05195 (WOC73_05195) - 1062478..1063464 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=971430 WOC73_RS05155 WP_029694050.1 1058011..1058508(-) (comGF) [Staphylococcus aureus strain TUM22730]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=971430 WOC73_RS05155 WP_029694050.1 1058011..1058508(-) (comGF) [Staphylococcus aureus strain TUM22730]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97

Multiple sequence alignment