Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   WJM45_RS03700 Genome accession   NZ_CP150634
Coordinates   832484..833545 (+) Length   353 a.a.
NCBI ID   WP_341327638.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 827484..838545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM45_RS03695 (WJM45_03695) - 829242..831614 (-) 2373 WP_341327637.1 penicillin-binding protein 1A -
  WJM45_RS03700 (WJM45_03700) pilM 832484..833545 (+) 1062 WP_341327638.1 pilus assembly protein PilM Machinery gene
  WJM45_RS03705 (WJM45_03705) - 833545..834108 (+) 564 WP_341327639.1 PilN domain-containing protein -
  WJM45_RS03710 (WJM45_03710) pilO 834108..834743 (+) 636 WP_341327640.1 type 4a pilus biogenesis protein PilO -
  WJM45_RS03715 (WJM45_03715) - 834740..835336 (+) 597 WP_341327641.1 pilus assembly protein PilP -
  WJM45_RS03720 (WJM45_03720) pilQ 835377..837656 (+) 2280 WP_341327642.1 type IV pilus secretin PilQ -
  WJM45_RS03725 (WJM45_03725) aroK 837734..838252 (+) 519 WP_341327643.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38391.86 Da        Isoelectric Point: 4.7289

>NTDB_id=970742 WJM45_RS03700 WP_341327638.1 832484..833545(+) (pilM) [Methylotuvimicrobium sp. KM2]
MNWFSRKNVPVVGIDISTAAVKLLELSKNGPRYRVESYAVAPLPQDAVIDKNFANVDVISDAIKQAIKQSGTKVKHACVA
VAGSAVMTKIITMPANYSDEEVYEQIMVEADQYVPYALDEVNLDFEVQGKNAKNSEMVDVLLVASRRENVEDRIEALDKA
GLKAKIIDVEAFAMENAFALIADQLPGSSEGQTIAISDIGSTMATLNVIADGRTIYTREQGFGGRQLTDEIQRRYGLSYE
EAGLAKRHGGLPDNYINDVLEPFKKAMVQQIARSLQFFVSSSANRTIDSIILAGGCSSIPGVDKLVEQNLSIPCFIANPF
INMALSNRVKPQSLSSDAPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=970742 WJM45_RS03700 WP_341327638.1 832484..833545(+) (pilM) [Methylotuvimicrobium sp. KM2]
ATGAACTGGTTTAGTCGCAAAAATGTGCCTGTAGTCGGTATCGACATTAGCACGGCAGCCGTTAAATTATTGGAATTATC
GAAAAACGGTCCTCGTTATCGAGTCGAAAGTTATGCCGTCGCGCCATTACCTCAGGATGCCGTTATCGATAAAAATTTCG
CGAATGTCGATGTTATCTCTGACGCCATCAAACAAGCCATCAAACAATCCGGAACGAAAGTTAAGCATGCCTGCGTCGCG
GTTGCGGGTTCGGCGGTTATGACCAAAATCATAACGATGCCTGCTAATTATTCGGACGAAGAAGTTTACGAACAAATCAT
GGTCGAGGCAGATCAATATGTGCCTTATGCACTAGATGAGGTCAACCTGGACTTTGAAGTTCAGGGAAAAAACGCTAAAA
ACTCCGAAATGGTTGATGTGCTCTTGGTCGCCTCCCGTCGTGAAAATGTCGAGGACCGCATCGAAGCCTTGGATAAGGCG
GGTTTAAAAGCCAAAATCATCGATGTCGAAGCGTTTGCGATGGAAAACGCCTTTGCTCTTATCGCCGATCAATTACCCGG
TTCTAGCGAAGGTCAGACTATTGCTATCTCGGATATCGGTTCGACGATGGCGACTTTGAACGTCATTGCGGACGGTAGAA
CCATTTATACGCGTGAACAAGGCTTCGGCGGAAGGCAGTTGACCGATGAAATTCAACGCCGCTACGGCTTGTCCTACGAA
GAAGCCGGTCTTGCCAAGCGGCATGGCGGTCTTCCCGATAATTATATCAATGACGTATTAGAGCCGTTTAAGAAGGCTAT
GGTTCAGCAAATCGCGCGATCACTCCAATTTTTTGTTTCATCGAGCGCCAATAGAACGATCGATTCGATTATTTTGGCGG
GCGGCTGCTCATCGATACCCGGTGTCGACAAGTTGGTCGAGCAGAACTTGAGTATTCCCTGTTTTATTGCCAACCCGTTT
ATTAATATGGCTTTATCGAATCGAGTGAAACCGCAAAGTTTAAGTAGCGATGCGCCGGCGATGATGATCGCCTGCGGTCT
TGCGCTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.992

100

0.51
  comM Acinetobacter nosocomialis M2

50.708

100

0.507
  comM Acinetobacter baylyi ADP1

48.442

100

0.484
  pilM Legionella pneumophila strain ERS1305867

41.076

100

0.411

Multiple sequence alignment