Detailed information NTG0970740

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WJM45_RS02335
• Genome accession: NZ_CP150634
• Coordinates: 525595..526758 (-)
• Length: 387 a.a.
• NCBI ID: WP_341327395.1
• Uniprot ID: -
• Organism: Methylotuvimicrobium sp. KM2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 520595..531758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WJM45_RS02320 (WJM45_02320)	-	520617..521759 (-)	1143	WP_341327392.1	thiolase family protein	-
WJM45_RS02325 (WJM45_02325)	-	521756..524095 (-)	2340	WP_341327393.1	alpha/beta fold hydrolase	-
WJM45_RS02330 (WJM45_02330)	-	524558..525049 (+)	492	WP_341327394.1	hypothetical protein	-
WJM45_RS02335 (WJM45_02335)	pilU	525595..526758 (-)	1164	WP_341327395.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WJM45_RS02340 (WJM45_02340)	-	526944..527987 (+)	1044	WP_341327396.1	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase	-
WJM45_RS02345 (WJM45_02345)	-	527984..528211 (+)	228	WP_341327397.1	sulfurtransferase TusA family protein	-
WJM45_RS02350 (WJM45_02350)	-	528211..529353 (+)	1143	WP_341327398.1	cation diffusion facilitator family transporter	-
WJM45_RS02355 (WJM45_02355)	argJ	529364..530578 (+)	1215	WP_341327399.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42772.02 Da
• Isoelectric Point: 6.2945

>NTDB_id=970740 WJM45_RS02335 WP_341327395.1 525595..526758(-) (pilU) [Methylotuvimicrobium sp. KM2]
MDFKDYLKILVSKDGSDLYLTVGAPAAAKFQGALKPLESEKLTGERIKAIAYSLMDADQQKDFEHVPEMNLAITEPGIGR
FRVNIFKQRNNLALVIRNIKVDIPNADQLGLPEVLKKVIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYIHPHKRCLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKAFVSQRLVPTVDGKRVAAIEILLGTQLVKDLIHKGEIHAIKEAMEKSENIGMQTFDSHL
LKLFKEGKISLEEALQNSDSPNNLKLKINLSEGKGSATEAVAKSPGGLTSRFSLEAIEKDQEEEGQS

Nucleotide

• Length: 1164 bp

>NTDB_id=970740 WJM45_RS02335 WP_341327395.1 525595..526758(-) (pilU) [Methylotuvimicrobium sp. KM2]
ATGGATTTTAAAGATTATCTAAAAATTCTAGTCAGCAAAGACGGCTCCGATCTTTACCTGACGGTCGGCGCGCCGGCCGC
GGCCAAATTTCAAGGCGCGCTGAAACCGCTCGAAAGCGAAAAACTGACCGGCGAACGCATCAAGGCTATCGCCTACAGTT
TAATGGATGCCGATCAGCAAAAAGACTTCGAACACGTCCCGGAAATGAATCTGGCGATCACCGAACCCGGCATCGGCCGC
TTCCGCGTCAATATTTTCAAACAGCGCAACAACTTGGCCTTGGTCATCCGCAACATCAAGGTCGATATTCCGAATGCCGA
TCAATTGGGCCTGCCTGAAGTGTTGAAAAAAGTCATCATGGAAAAGCGCGGTTTGATTCTGTTCGTCGGGGGTACCGGCT
CCGGCAAGTCGACCTCCCTGGCCGCACTGATCGATCACCGCAACAGTAACTCGTCGGGGCACATCATCACGATAGAAGAC
CCGATCGAATACATACATCCGCACAAACGCTGCCTGGTCAATCAACGCGAAGTCGGCGTCGATACGCTCAGCTACGAAGA
CGCGCTGAAAAACACGCTTCGCCAAGCTCCCGACGTGATTCTGATCGGCGAAATCCGCTCTCAAGAAACGATGGAACACG
CGCTCGCCTTCGCCGAAACCGGACATCTTTGTTTATCGACGCTGCATGCCAACAACGCCAATCAGGCTTTGGACCGCATC
ATCAATTTTTTCCCGGAAGAGCGGCGTAACCAATTATTGCTGGATCTATCGCTGAATTTGAAAGCCTTTGTTTCGCAAAG
ACTGGTTCCTACCGTGGACGGCAAACGGGTCGCGGCGATCGAAATTTTGCTGGGAACACAATTGGTCAAAGACTTGATTC
ATAAAGGCGAAATTCATGCGATCAAGGAAGCGATGGAAAAATCCGAAAACATCGGCATGCAAACCTTCGATAGCCATTTG
CTCAAACTTTTCAAGGAAGGCAAAATTTCGCTCGAAGAAGCTTTGCAAAATTCCGATTCGCCGAACAATCTGAAATTAAA
AATCAACCTCAGCGAAGGCAAGGGTTCGGCGACCGAAGCGGTCGCTAAATCGCCTGGCGGACTGACTTCAAGATTCTCGC
TTGAGGCGATTGAAAAGGATCAGGAGGAAGAAGGACAAAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.267	96.899	0.545
pilU	Acinetobacter baylyi ADP1	54.237	91.473	0.496
pilU	Vibrio cholerae strain A1552	50.847	91.473	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.417	94.832	0.393