Detailed information NTG0970353

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACLFK2_RS02185
• Genome accession: NZ_CP177307
• Coordinates: 468317..469465 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Pa021
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 463317..474465

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLFK2_RS02160 (ACLFK2_02160)	-	464468..464764 (-)	297	WP_031627661.1	DUF167 domain-containing protein	-
ACLFK2_RS02165 (ACLFK2_02165)	-	464761..465354 (-)	594	WP_003084544.1	YggT family protein	-
ACLFK2_RS02170 (ACLFK2_02170)	proC	465365..466186 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACLFK2_RS02175 (ACLFK2_02175)	-	466198..466890 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACLFK2_RS02180 (ACLFK2_02180)	pilT	467105..468139 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACLFK2_RS02185 (ACLFK2_02185)	pilU	468317..469465 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACLFK2_RS02190 (ACLFK2_02190)	czcD	469473..470372 (-)	900	WP_003162016.1	CDF family zinc transporter CzcD	-
ACLFK2_RS02195 (ACLFK2_02195)	-	470526..470930 (+)	405	WP_003084560.1	NINE protein	-
ACLFK2_RS02200 (ACLFK2_02200)	-	471173..472546 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACLFK2_RS02205 (ACLFK2_02205)	-	472543..473727 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=970353 ACLFK2_RS02185 WP_003100959.1 468317..469465(+) (pilU) [Pseudomonas aeruginosa strain Pa021]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=970353 ACLFK2_RS02185 WP_003100959.1 468317..469465(+) (pilU) [Pseudomonas aeruginosa strain Pa021]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382