Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AACH74_RS10180 Genome accession   NZ_AP029000
Coordinates   2133465..2134475 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain GU2018_CL13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2128465..2139475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH74_RS10150 (GU2018CL13_19600) nudB 2130188..2130640 (+) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -
  AACH74_RS10155 (GU2018CL13_19610) yebC 2130669..2131409 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AACH74_RS10160 (GU2018CL13_19620) ruvC 2131444..2131965 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  AACH74_RS10165 (GU2018CL13_19630) yebB 2131967..2132569 (-) 603 WP_001024939.1 YebB family permuted papain-like enzyme -
  AACH74_RS10170 yobI 2132641..2132706 (+) 66 WP_072123646.1 stress response small protein YobI -
  AACH74_RS10175 (GU2018CL13_19640) ruvA 2132845..2133456 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  AACH74_RS10180 (GU2018CL13_19650) ruvB 2133465..2134475 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AACH74_RS10185 (GU2018CL13_19660) znuB 2134692..2135477 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  AACH74_RS10190 (GU2018CL13_19670) znuC 2135474..2136229 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  AACH74_RS10195 (GU2018CL13_19680) znuA 2136308..2137240 (+) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -
  AACH74_RS10200 (GU2018CL13_19690) mepM 2137256..2138578 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=97003 AACH74_RS10180 WP_000568519.1 2133465..2134475(+) (ruvB) [Escherichia coli strain GU2018_CL13]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=97003 AACH74_RS10180 WP_000568519.1 2133465..2134475(+) (ruvB) [Escherichia coli strain GU2018_CL13]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment