Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MKX80_RS14180 Genome accession   NZ_CP150260
Coordinates   2805452..2806081 (-) Length   209 a.a.
NCBI ID   WP_154994395.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0972     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2800452..2811081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX80_RS14160 (MKX80_14160) holA 2801012..2802037 (-) 1026 WP_182070346.1 DNA polymerase III subunit delta -
  MKX80_RS14165 (MKX80_14165) - 2802267..2802398 (+) 132 WP_003183686.1 YqzM family protein -
  MKX80_RS14170 (MKX80_14170) - 2802423..2804810 (-) 2388 WP_268343013.1 DNA internalization-related competence protein ComEC/Rec2 -
  MKX80_RS14175 (MKX80_14175) - 2804815..2805384 (-) 570 WP_234112005.1 ComE operon protein 2 -
  MKX80_RS14180 (MKX80_14180) comEA 2805452..2806081 (-) 630 WP_154994395.1 helix-hairpin-helix domain-containing protein Machinery gene
  MKX80_RS14185 (MKX80_14185) comER 2806164..2806985 (+) 822 WP_217903537.1 late competence protein ComER -
  MKX80_RS14190 (MKX80_14190) - 2807055..2807810 (-) 756 WP_154994399.1 class I SAM-dependent methyltransferase -
  MKX80_RS14195 (MKX80_14195) rsfS 2807807..2808163 (-) 357 WP_003183699.1 ribosome silencing factor -
  MKX80_RS14200 (MKX80_14200) yqeK 2808177..2808740 (-) 564 WP_268343012.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MKX80_RS14205 (MKX80_14205) - 2808730..2809299 (-) 570 WP_154994403.1 nicotinate-nucleotide adenylyltransferase -
  MKX80_RS14210 (MKX80_14210) yhbY 2809318..2809608 (-) 291 WP_268316148.1 ribosome assembly RNA-binding protein YhbY -
  MKX80_RS14215 (MKX80_14215) aroE 2809602..2810438 (-) 837 WP_154994407.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22402.28 Da        Isoelectric Point: 5.0202

>NTDB_id=968468 MKX80_RS14180 WP_154994395.1 2805452..2806081(-) (comEA) [Bacillus sp. FSL K6-0972]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSFPEEASAQTFDKKEEAKREKNAGKEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTVKKADQKQINLAAVLQDGMVVYIPFEGEETAGSLSEAGSRAEGNSGGLVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=968468 MKX80_RS14180 WP_154994395.1 2805452..2806081(-) (comEA) [Bacillus sp. FSL K6-0972]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCCGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCGGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCTTTTCCTGAGGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGCGAAGCGGGAAAAAAACGCGGGGAAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGTGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGTGAAAAAAGCGGACCAAAAACA
AATTAATCTGGCAGCCGTTTTGCAGGACGGTATGGTTGTTTACATTCCATTTGAAGGGGAAGAGACAGCTGGTTCTCTTT
CGGAAGCGGGCTCAAGAGCAGAAGGCAATTCCGGTGGTCTCGTCAATATTAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCAGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGTGAGGAGAACGGACCGTTTCAGACGATCGAAGA
CATCACGAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTTCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-123)

Helix-hairpin-helix motif (HHH_3)

(146-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

55.14

100

0.565
  comEA Staphylococcus aureus N315

35.455

100

0.373
  comEA Staphylococcus aureus MW2

35.455

100

0.373
  comEA Lactococcus lactis subsp. cremoris KW2

35.945

100

0.373

Multiple sequence alignment