Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   MKY32_RS14120 Genome accession   NZ_CP150259
Coordinates   2675938..2676231 (-) Length   97 a.a.
NCBI ID   WP_003183466.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0998     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2670938..2681231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY32_RS14075 (MKY32_14075) - 2671320..2672114 (-) 795 WP_003183447.1 TasA family protein -
  MKY32_RS14080 (MKY32_14080) - 2672188..2672772 (-) 585 WP_003183449.1 signal peptidase I -
  MKY32_RS14085 (MKY32_14085) tapA 2672769..2673497 (-) 729 WP_003183451.1 amyloid fiber anchoring/assembly protein TapA -
  MKY32_RS14090 (MKY32_14090) - 2673807..2674094 (+) 288 WP_223307118.1 YqzG/YhdC family protein -
  MKY32_RS14095 (MKY32_14095) - 2674118..2674300 (-) 183 WP_003183456.1 YqzE family protein -
  MKY32_RS14100 (MKY32_14100) comGG 2674389..2674754 (-) 366 WP_339243778.1 competence type IV pilus minor pilin ComGG -
  MKY32_RS14105 (MKY32_14105) comGF 2674767..2675255 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  MKY32_RS14110 (MKY32_14110) comGE 2675164..2675511 (-) 348 WP_009327907.1 competence type IV pilus minor pilin ComGE -
  MKY32_RS14115 (MKY32_14115) comGD 2675495..2675932 (-) 438 WP_118381805.1 competence type IV pilus minor pilin ComGD -
  MKY32_RS14120 (MKY32_14120) comGC 2675938..2676231 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  MKY32_RS14125 (MKY32_14125) comGB 2676245..2677279 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  MKY32_RS14130 (MKY32_14130) comGA 2677269..2678333 (-) 1065 WP_003183477.1 competence type IV pilus ATPase ComGA Machinery gene
  MKY32_RS14135 (MKY32_14135) - 2678490..2679329 (-) 840 WP_003183480.1 STAS domain-containing protein -
  MKY32_RS14145 (MKY32_14145) - 2679571..2679948 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKY32_RS14150 (MKY32_14150) - 2680157..2680402 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  MKY32_RS14155 (MKY32_14155) - 2680440..2681078 (-) 639 WP_003183485.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10725.62 Da        Isoelectric Point: 9.1200

>NTDB_id=968389 MKY32_RS14120 WP_003183466.1 2675938..2676231(-) (comGC) [Bacillus sp. FSL K6-0998]
MNEKGFTLIEMLVVMLVISILLLITIPNVTKHNQSIQKKGCEGLINMVQAQITAYQMDHDGKTPNRAELESEGYIKKNLK
CPNGKAIKISNGKAQAD

Nucleotide


Download         Length: 294 bp        

>NTDB_id=968389 MKY32_RS14120 WP_003183466.1 2675938..2676231(-) (comGC) [Bacillus sp. FSL K6-0998]
GTGAATGAAAAGGGATTTACACTGATAGAAATGTTAGTCGTCATGCTCGTTATCTCCATCCTGCTGTTGATCACCATTCC
GAATGTGACCAAGCATAACCAAAGCATCCAAAAGAAAGGATGCGAAGGATTAATCAATATGGTTCAAGCGCAGATCACGG
CCTATCAGATGGATCATGACGGGAAAACGCCGAACAGGGCGGAGCTTGAATCTGAAGGTTACATAAAAAAGAACTTAAAG
TGTCCGAATGGGAAAGCAATTAAAATTTCAAACGGCAAAGCACAGGCTGATTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

68.041

100

0.68
  comGC Staphylococcus aureus MW2

44.211

97.938

0.433
  comGC Staphylococcus aureus N315

44.211

97.938

0.433

Multiple sequence alignment