Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   MKY32_RS14125 Genome accession   NZ_CP150259
Coordinates   2676245..2677279 (-) Length   344 a.a.
NCBI ID   WP_011198115.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0998     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2671245..2682279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY32_RS14075 (MKY32_14075) - 2671320..2672114 (-) 795 WP_003183447.1 TasA family protein -
  MKY32_RS14080 (MKY32_14080) - 2672188..2672772 (-) 585 WP_003183449.1 signal peptidase I -
  MKY32_RS14085 (MKY32_14085) tapA 2672769..2673497 (-) 729 WP_003183451.1 amyloid fiber anchoring/assembly protein TapA -
  MKY32_RS14090 (MKY32_14090) - 2673807..2674094 (+) 288 WP_223307118.1 YqzG/YhdC family protein -
  MKY32_RS14095 (MKY32_14095) - 2674118..2674300 (-) 183 WP_003183456.1 YqzE family protein -
  MKY32_RS14100 (MKY32_14100) comGG 2674389..2674754 (-) 366 WP_339243778.1 competence type IV pilus minor pilin ComGG -
  MKY32_RS14105 (MKY32_14105) comGF 2674767..2675255 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  MKY32_RS14110 (MKY32_14110) comGE 2675164..2675511 (-) 348 WP_009327907.1 competence type IV pilus minor pilin ComGE -
  MKY32_RS14115 (MKY32_14115) comGD 2675495..2675932 (-) 438 WP_118381805.1 competence type IV pilus minor pilin ComGD -
  MKY32_RS14120 (MKY32_14120) comGC 2675938..2676231 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  MKY32_RS14125 (MKY32_14125) comGB 2676245..2677279 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  MKY32_RS14130 (MKY32_14130) comGA 2677269..2678333 (-) 1065 WP_003183477.1 competence type IV pilus ATPase ComGA Machinery gene
  MKY32_RS14135 (MKY32_14135) - 2678490..2679329 (-) 840 WP_003183480.1 STAS domain-containing protein -
  MKY32_RS14145 (MKY32_14145) - 2679571..2679948 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MKY32_RS14150 (MKY32_14150) - 2680157..2680402 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  MKY32_RS14155 (MKY32_14155) - 2680440..2681078 (-) 639 WP_003183485.1 MBL fold metallo-hydrolase -
  MKY32_RS14160 (MKY32_14160) - 2681234..2681407 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  MKY32_RS14165 (MKY32_14165) - 2681468..2681782 (-) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39737.95 Da        Isoelectric Point: 9.3543

>NTDB_id=968390 MKY32_RS14125 WP_011198115.1 2676245..2677279(-) (comGB) [Bacillus sp. FSL K6-0998]
MKIKMKWPVREQADLLKKLGDMMQSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGCPVFQVLEMISFHKDAVTIVYF
AEQHGNLPFAFRQSGELLHRKIAQSEKLKKAARYPLFLVLTVCVIISIMKSAIVPQFSAIYESMNVETSFATTLIFYVFD
HFYLFFAGLPLVAAALSLYYLCSFRHKPPEDKMAFLITIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LRFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=968390 MKY32_RS14125 WP_011198115.1 2676245..2677279(-) (comGB) [Bacillus sp. FSL K6-0998]
ATGAAGATTAAAATGAAGTGGCCTGTCCGGGAACAGGCGGACCTTCTGAAAAAACTAGGAGACATGATGCAGAGTGGCTA
TACGATTTTGGATGCTTTAAGTATGCTGAAATTGCAATTGAATGAAAGACGAAGAGCGGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGTGTCCTGTGTTTCAAGTTCTTGAAATGATTTCCTTTCACAAGGATGCTGTCACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATTTGCCTTTAGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAAGCGAAAA
ATTAAAGAAAGCCGCCAGATACCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATCATCTCTATTATGAAATCAGCAATAG
TTCCGCAATTTTCCGCCATCTATGAATCGATGAACGTTGAAACGTCTTTTGCAACGACTCTCATCTTTTATGTTTTCGAT
CACTTTTATCTTTTTTTCGCGGGACTGCCGCTGGTTGCCGCAGCTCTATCCCTCTATTATCTGTGTTCATTCCGCCATAA
ACCTCCAGAAGACAAAATGGCCTTTCTCATTACAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGCTATTTTT
TATCGCTCCAGCTGAGCAATCTTTTGCAAGCGGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTACGATTTCATAAAAATGAAGCGAAGCGCTTGATCGAGCGGCTGAAACAGGGGGAGAGTCTGGAGCAGATGCTGGCCGG
ACATCCGTTTTATGAAAATGATTTAGCAAAGGCTGTTGCACACGGCCAACTAAATGGGCACTTATACAGAGAGCTGTATT
CATATAGTCAGTTTTTGATTGAGCGGCTTGAAAGAAAGGCCGAAAAATGGACAGGCCTGATCCAGCCATTGATTTACGGG
CTAACCGCGGCGATGATATTAATTCTTTATTTGTCCATGCTTTTGCCAATGTATCAAATGATGAACCAGCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

56.656

93.895

0.532

Multiple sequence alignment