Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OO778_RS01675 Genome accession   NZ_AP026765
Coordinates   354439..355455 (+) Length   338 a.a.
NCBI ID   WP_164667087.1    Uniprot ID   -
Organism   Vibrio sp. STUT-A16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 349439..360455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO778_RS01660 (VspSTUT16_02800) - 349872..350600 (-) 729 WP_005395721.1 redoxin family protein -
  OO778_RS01665 (VspSTUT16_02810) oxyR 350771..351682 (+) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  OO778_RS01670 (VspSTUT16_02820) - 351734..354295 (-) 2562 WP_264910641.1 PBP1A family penicillin-binding protein -
  OO778_RS01675 (VspSTUT16_02830) pilM 354439..355455 (+) 1017 WP_164667087.1 type IV pilus assembly protein PilM Machinery gene
  OO778_RS01680 (VspSTUT16_02840) pilN 355439..356014 (+) 576 WP_074191323.1 PilN domain-containing protein Machinery gene
  OO778_RS01685 (VspSTUT16_02850) pilO 356007..356600 (+) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  OO778_RS01690 (VspSTUT16_02860) pilP 356590..357105 (+) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  OO778_RS01695 (VspSTUT16_02870) pilQ 357137..358879 (+) 1743 WP_080955983.1 type IV pilus secretin PilQ family protein Machinery gene
  OO778_RS01700 (VspSTUT16_02880) aroK 359067..359585 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37677.05 Da        Isoelectric Point: 6.9710

>NTDB_id=96833 OO778_RS01675 WP_164667087.1 354439..355455(+) (pilM) [Vibrio sp. STUT-A16]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECDLLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=96833 OO778_RS01675 WP_164667087.1 354439..355455(+) (pilM) [Vibrio sp. STUT-A16]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAACTGCAAATAGAACAAAATCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCGTTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGTGAGCTATTTCACAAAGAGTTTGCTT
GCGGCACTCGAGGAGCAAACAGCCCTGTTCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGATTTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.249

100

0.822
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5

Multiple sequence alignment