Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NSQ78_RS17420 Genome accession   NZ_CP150199
Coordinates   3740697..3741755 (-) Length   352 a.a.
NCBI ID   WP_339206970.1    Uniprot ID   -
Organism   Stenotrophomonas sp. FSL W7-1447     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3735697..3746755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ78_RS17400 (NSQ78_17400) - 3736641..3738611 (-) 1971 WP_064239107.1 type IV pilus secretin PilQ family protein -
  NSQ78_RS17405 (NSQ78_17405) - 3738633..3739166 (-) 534 WP_005418914.1 pilus assembly protein PilP -
  NSQ78_RS17410 (NSQ78_17410) pilO 3739166..3739822 (-) 657 WP_057493352.1 type 4a pilus biogenesis protein PilO -
  NSQ78_RS17415 (NSQ78_17415) - 3739819..3740697 (-) 879 WP_053506172.1 PilN domain-containing protein -
  NSQ78_RS17420 (NSQ78_17420) pilM 3740697..3741755 (-) 1059 WP_339206970.1 pilus assembly protein PilM Machinery gene
  NSQ78_RS17425 (NSQ78_17425) - 3741975..3744398 (+) 2424 WP_053461282.1 penicillin-binding protein 1A -
  NSQ78_RS17430 (NSQ78_17430) - 3744582..3745226 (+) 645 WP_049465702.1 hypothetical protein -
  NSQ78_RS17435 (NSQ78_17435) - 3745342..3746085 (-) 744 WP_057493350.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37755.38 Da        Isoelectric Point: 4.4006

>NTDB_id=966063 NSQ78_RS17420 WP_339206970.1 3740697..3741755(-) (pilM) [Stenotrophomonas sp. FSL W7-1447]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPADLDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGSIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIDGVVALIDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=966063 NSQ78_RS17420 WP_339206970.1 3740697..3741755(-) (pilM) [Stenotrophomonas sp. FSL W7-1447]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTCGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGCGGCAATCGTTTCCGCGTGGAACATTACGCTGTGGAACCTCTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TCGAGGTGGAGGCCGTGGGTGAGGCCATCCGCCGGGCGATGAACCGTTCAGGCAGCAAGGCCAAGCTGGCCGCTGCCGCC
GTCGCGGGGTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGACCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCCTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGTCGATCCCGAACA
ACCCGGAGATGGTCCAAGTGCTGCTGGCGGCCTCGCGTTCGGAGAATGTCGAGCTGCGCCAGTCCGCGCTGGAACTGGGC
GGACTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTACGCCCTGGTCGCCAGCGAACTGCCGGT
GTCCATCGACGGCGTGGTCGCGCTGATCGACATCGGCGCCACCATGACCACCCTCAATGTCCTGCGCGGCGGCCGTAGCC
TCTACAGCCGCGAGCAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTACGGGCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGCCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTATGCAGGCAGCGAATTCAACCGCGTCGATCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTCGGTGGCCTGCCGGAGATGGTCGAGGAACAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCCCATGCGTTGGCCCAGGATGCCCCCGCGCTGATGATCGCGACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.578

99.716

0.474

Multiple sequence alignment