Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NST56_RS13720 Genome accession   NZ_CP150195
Coordinates   2587464..2588534 (-) Length   356 a.a.
NCBI ID   WP_168748237.1    Uniprot ID   A0AAP3CRF1
Organism   Bacillus sp. FSL R5-0560     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2582464..2593534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST56_RS13675 (NST56_13675) tapA 2582939..2583700 (-) 762 WP_339177150.1 amyloid fiber anchoring/assembly protein TapA -
  NST56_RS13680 (NST56_13680) - 2583977..2584300 (+) 324 WP_168748230.1 YqzG/YhdC family protein -
  NST56_RS13685 (NST56_13685) - 2584343..2584522 (-) 180 WP_003236949.1 YqzE family protein -
  NST56_RS13690 (NST56_13690) comGG 2584594..2584968 (-) 375 WP_339177153.1 competence type IV pilus minor pilin ComGG Machinery gene
  NST56_RS13695 (NST56_13695) comGF 2584969..2585352 (-) 384 WP_339177156.1 competence type IV pilus minor pilin ComGF Machinery gene
  NST56_RS13700 (NST56_13700) comGE 2585378..2585725 (-) 348 WP_168748233.1 competence type IV pilus minor pilin ComGE Machinery gene
  NST56_RS13705 (NST56_13705) comGD 2585709..2586140 (-) 432 WP_168748234.1 competence type IV pilus minor pilin ComGD Machinery gene
  NST56_RS13710 (NST56_13710) comGC 2586130..2586426 (-) 297 WP_168748235.1 competence type IV pilus major pilin ComGC Machinery gene
  NST56_RS13715 (NST56_13715) comGB 2586440..2587477 (-) 1038 WP_326191101.1 competence type IV pilus assembly protein ComGB Machinery gene
  NST56_RS13720 (NST56_13720) comGA 2587464..2588534 (-) 1071 WP_168748237.1 competence protein ComGA Machinery gene
  NST56_RS13725 (NST56_13725) - 2588887..2589057 (-) 171 WP_339177165.1 hypothetical protein -
  NST56_RS13730 (NST56_13730) - 2589281..2590231 (-) 951 WP_010334929.1 magnesium transporter CorA family protein -
  NST56_RS13735 (NST56_13735) - 2590375..2591704 (+) 1330 Protein_2659 hemolysin family protein -
  NST56_RS13740 (NST56_13740) - 2591784..2592623 (-) 840 WP_268451132.1 STAS domain-containing protein -
  NST56_RS13750 (NST56_13750) mgsR 2592856..2593236 (-) 381 WP_026014741.1 transcriptional regulator MgsR -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40445.55 Da        Isoelectric Point: 8.6434

>NTDB_id=965856 NST56_RS13720 WP_168748237.1 2587464..2588534(-) (comGA) [Bacillus sp. FSL R5-0560]
MDSIENISRTLIEEAYKTKASDIHIVPRERDAIIHFRVDNALLKKRNMKKEECVRLISHFKFLSAMDIGERRKPQSGSLT
LKLKEGDVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPQTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLIQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAQIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPYCENGCSSVYCRLSRNIRRASVYELLYGKNLQQCIQEAKGD
NANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHETD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=965856 NST56_RS13720 WP_168748237.1 2587464..2588534(-) (comGA) [Bacillus sp. FSL R5-0560]
TTGGATTCGATAGAAAACATAAGTAGAACCTTAATAGAAGAGGCATATAAGACGAAGGCCTCTGACATTCACATTGTGCC
AAGGGAACGGGACGCGATCATCCATTTTCGGGTTGACAATGCATTGCTGAAAAAGCGAAACATGAAAAAAGAAGAGTGTG
TAAGGCTCATTTCTCATTTTAAGTTTCTCTCAGCTATGGACATTGGGGAGAGAAGAAAGCCGCAAAGTGGATCGCTTACT
CTAAAATTAAAAGAGGGTGACGTTCACTTAAGGATGTCAACGCTCCCGACAATTAACGAAGAAAGCCTCGTCATCAGAGT
GATGCCTCAGTATAATATCCCTTCGATTGACAAACTGTCTTTATTCCCACAGACAGGAGCCACCTTGCTTTCATTTTTAA
AACACTCCCACGGCATGCTGATTTTTACCGGACCAACAGGCTCTGGGAAAACAACAACGTTATATTCTCTGATCCAATAT
GCAAAAAAACATTTTAATCGAAACATTGTCACACTTGAAGATCCAGTGGAAACGAGGGATGAAGATGTTCTTCAGGTGCA
GGTGAATGAAAAAGCCGGGGTCACATATTCCACAGGGCTGAAGGCTATTTTGCGCCATGACCCCGATATGATCATTTTAG
GTGAGATTAGAGATGCTGAAACAGCCCAAATCGCGGTGCGGGCAGCGATGACTGGTCATTTAGTGCTGACGAGCCTTCAT
ACGAGAGATGCCAAGGGCGCGATTTACAGGCTGCTTGAATTTGGCATTAATATGAATGAAATCGAGCAAACGGTTATTGC
GATAGCGGCTCAGCGTTTAGTTGATTTGGCTTGCCCGTATTGTGAAAACGGATGTTCTTCAGTGTATTGCCGACTGTCAC
GAAACATAAGGAGAGCAAGCGTATACGAGCTGCTATACGGGAAAAACCTTCAGCAGTGTATTCAGGAAGCGAAAGGGGAC
AATGCAAATTACCAATATCAAACGTTGCGTCAAATTATAAGAAAAGGGATTGCGCTCGGCTATTTAACGACAAATAACTA
TGACCGGTGGGTTTATCATGAAACAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

94.663

100

0.947
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.283

97.191

0.362

Multiple sequence alignment