Detailed information NTG0962844

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WHK83_RS13945
• Genome accession: NZ_CP150097
• Coordinates: 2971776..2972894 (+)
• Length: 372 a.a.
• NCBI ID: WP_406859370.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain HAU425
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2966776..2977894

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHK83_RS13935	-	2969892..2970584 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
WHK83_RS13940	pilT	2970711..2971748 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
WHK83_RS13945	pilU	2971776..2972894 (+)	1119	WP_406859370.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WHK83_RS13950	fur	2972964..2973401 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
WHK83_RS13955	-	2973513..2973911 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
WHK83_RS13960	-	2973966..2974289 (-)	324	WP_000442589.1	RnfH family protein	-
WHK83_RS13965	-	2974289..2975356 (-)	1068	WP_004748097.1	hypothetical protein	-
WHK83_RS13970	-	2975509..2975964 (-)	456	WP_000782976.1	bacteriohemerythrin	-
WHK83_RS13975	-	2976217..2977065 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41918.16 Da
• Isoelectric Point: 6.8289

>NTDB_id=962844 WHK83_RS13945 WP_406859370.1 2971776..2972894(+) (pilU) [Acinetobacter baumannii strain HAU425]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
HPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=962844 WHK83_RS13945 WP_406859370.1 2971776..2972894(+) (pilU) [Acinetobacter baumannii strain HAU425]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
CACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.75	98.925	0.68
pilU	Vibrio cholerae strain A1552	52.703	99.462	0.524
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39