Detailed information NTG0961458

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WF513_RS08150
• Genome accession: NZ_CP149803
• Coordinates: 1718645..1719871 (+)
• Length: 408 a.a.
• NCBI ID: WP_339083128.1
• Uniprot ID: -
• Organism: Pseudomonas sp. TMP9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1713645..1724871

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WF513_RS08120 (WF513_08120)	-	1714471..1715499 (-)	1029	WP_339080618.1	IS30 family transposase	-
WF513_RS08125 (WF513_08125)	-	1715656..1716105 (+)	450	WP_339083120.1	SMI1/KNR4 family protein	-
WF513_RS08130 (WF513_08130)	-	1716224..1716850 (-)	627	Protein_1574	IS30 family transposase	-
WF513_RS08135 (WF513_08135)	-	1716922..1717620 (+)	699	WP_339083122.1	transposase	-
WF513_RS08140 (WF513_08140)	-	1717864..1718217 (-)	354	WP_339083124.1	MmcQ/YjbR family DNA-binding protein	-
WF513_RS08145 (WF513_08145)	-	1718224..1718502 (-)	279	WP_339083126.1	peptidylprolyl isomerase	-
WF513_RS08150 (WF513_08150)	pilU	1718645..1719871 (+)	1227	WP_339083128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WF513_RS08155 (WF513_08155)	-	1719914..1721869 (-)	1956	WP_339083129.1	acetoacetate--CoA ligase	-
WF513_RS08160 (WF513_08160)	-	1722268..1723170 (-)	903	WP_339083131.1	hydroxymethylglutaryl-CoA lyase	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 44668.08 Da
• Isoelectric Point: 6.1330

>NTDB_id=961458 WF513_RS08150 WP_339083128.1 1718645..1719871(+) (pilU) [Pseudomonas sp. TMP9]
MDLNAMLKVLSSQDGSDLYLSTGAPPCAKFNGVLKALSAEALKAGEVAAIAESVMDSAQREEFERELEMNLAISIAGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTIRDLIKRGDFSDIKEIMEKSKNLGMQTFDQAL
IDLVNDGSIDEEEAVKNADSANNVRLKLKLYRDNPTASVPVEAAVPAPVAAAPRPPAPAASAGEWGLELKLEDIEHDLPP
EDPGRKGI

Nucleotide

• Length: 1227 bp

>NTDB_id=961458 WF513_RS08150 WP_339083128.1 1718645..1719871(+) (pilU) [Pseudomonas sp. TMP9]
ATGGACCTCAATGCAATGCTCAAAGTCCTGTCCAGTCAGGACGGCTCCGACCTTTATCTGTCAACTGGTGCGCCGCCCTG
CGCTAAGTTCAACGGCGTGCTTAAGGCGCTGAGTGCCGAAGCGCTTAAGGCCGGCGAGGTGGCAGCAATCGCTGAGTCGG
TGATGGACAGTGCGCAGCGCGAAGAATTTGAGCGTGAGTTGGAGATGAACTTAGCGATTTCCATCGCCGGCGTTGGGCGT
TTCCGCATCAATATTTTTAAGCAGCGCAACGAAGTGTCGATTGTGGCGCGCAATATCAAGTTGGATATCCCCAAGTTCGA
AGACCTCAAACTGCCGGAGGTGTTGCTTAAAACCGTGATGGAAAAACGCGGCCTGGTGTTGTTTGTGGGCGGTACCGGCT
CAGGTAAGTCGACCTCGCTGGCGGCCTTAATCGATTACCGCAACCGCAACAGTGGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTATGTGCACAAGCACAAAAAGTCGATCATCAACCAGCGTGAAGTCGGGGTCGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGTCAGGCGCCGGATGTGATCTTAATTGGTGAAATTCGTGACCGTGAAACCATGGAGCATG
CGCTGGCGTTTGCTGACACCGGCCACCTGGCGATTTCCACGCTGCACGCCAACAACGCCAATCAAGCGCTGGACCGCATC
ATCAATTTTTTTCCGGAAGATCGTCGCCCGCAGTTGCTCAACGACCTGGGCAACAATCTCAAGGCGTTTGTTTCGCAGCG
GCTGGTGAAAACCGTCGACGGCAAGCGCCGGGCGGCAGTCGAGGTGCTGCTGGGCACGCCAACCATTCGTGACTTGATCA
AACGCGGTGATTTTAGCGATATCAAAGAGATCATGGAGAAATCCAAGAACCTCGGCATGCAAACCTTCGATCAGGCACTC
ATCGACTTAGTCAATGACGGTTCGATTGATGAAGAAGAAGCCGTTAAGAACGCCGATTCGGCCAACAACGTGCGCCTCAA
GCTTAAGCTTTATCGGGACAATCCCACCGCATCGGTGCCGGTTGAAGCAGCAGTCCCTGCGCCAGTTGCAGCTGCGCCGC
GGCCGCCAGCGCCCGCTGCTTCGGCCGGTGAGTGGGGCCTTGAGTTGAAGCTGGAAGATATTGAGCATGACCTGCCGCCC
GAAGATCCCGGGCGTAAGGGCATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (39-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	86.029	0.483
pilU	Vibrio cholerae strain A1552	51.811	87.99	0.456
pilU	Acinetobacter baylyi ADP1	52.557	86.275	0.453
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.66	95.098	0.368