Detailed information NTG0961427

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WKW51_RS14035
• Genome accession: NZ_CP149800
• Coordinates: 2931305..2932423 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB7276
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2926305..2937423

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKW51_RS14025 (WKW51_14025)	-	2929429..2930121 (-)	693	WP_001108504.1	YggS family pyridoxal phosphate-dependent enzyme	-
WKW51_RS14030 (WKW51_14030)	pilT	2930248..2931285 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
WKW51_RS14035 (WKW51_14035)	pilU	2931305..2932423 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WKW51_RS14040 (WKW51_14040)	fur	2932494..2932931 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
WKW51_RS14045 (WKW51_14045)	bamE	2933043..2933441 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
WKW51_RS14050 (WKW51_14050)	-	2933496..2933819 (-)	324	WP_000442589.1	RnfH family protein	-
WKW51_RS14055 (WKW51_14055)	-	2933819..2934886 (-)	1068	WP_002084046.1	hypothetical protein	-
WKW51_RS14060 (WKW51_14060)	-	2935039..2935494 (-)	456	WP_000782976.1	bacteriohemerythrin	-
WKW51_RS14065 (WKW51_14065)	-	2935747..2936595 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=961427 WKW51_RS14035 WP_000347036.1 2931305..2932423(+) (pilU) [Acinetobacter baumannii strain AB7276]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=961427 WKW51_RS14035 WP_000347036.1 2931305..2932423(+) (pilU) [Acinetobacter baumannii strain AB7276]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392