Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WKI54_RS16575 Genome accession   NZ_CP149648
Coordinates   3222458..3222892 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain MJ04     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3217458..3227892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI54_RS16565 (WKI54_16565) - 3217860..3220826 (+) 2967 Protein_3199 beta-ketoacyl synthase N-terminal-like domain-containing protein -
  WKI54_RS16570 (WKI54_16570) - 3220961..3222169 (-) 1209 WP_339075959.1 cytochrome P450 -
  WKI54_RS16575 (WKI54_16575) nucA/comI 3222458..3222892 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  WKI54_RS16580 (WKI54_16580) - 3222952..3223707 (+) 756 WP_003154084.1 YoaK family protein -
  WKI54_RS16585 (WKI54_16585) - 3223741..3224103 (-) 363 WP_007410383.1 hypothetical protein -
  WKI54_RS16590 (WKI54_16590) - 3224295..3225623 (-) 1329 WP_017417817.1 S8 family peptidase -
  WKI54_RS16595 (WKI54_16595) - 3225802..3226035 (+) 234 WP_106067946.1 hypothetical protein -
  WKI54_RS16600 (WKI54_16600) - 3226290..3226997 (+) 708 WP_014417828.1 poly-gamma-glutamate hydrolase family protein -
  WKI54_RS16605 (WKI54_16605) - 3227058..3227510 (+) 453 WP_110085567.1 OsmC family protein -
  WKI54_RS16610 (WKI54_16610) - 3227524..3227877 (-) 354 WP_029973435.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=960300 WKI54_RS16575 WP_007611576.1 3222458..3222892(+) (nucA/comI) [Bacillus velezensis strain MJ04]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=960300 WKI54_RS16575 WP_007611576.1 3222458..3222892(+) (nucA/comI) [Bacillus velezensis strain MJ04]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486