Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   WKI52_RS02350 Genome accession   NZ_CP149646
Coordinates   455114..456499 (-) Length   461 a.a.
NCBI ID   WP_063636495.1    Uniprot ID   -
Organism   Bacillus velezensis strain MJ06     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450114..461499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI52_RS02315 (WKI52_02315) flgL 450289..451206 (-) 918 WP_007407477.1 flagellar hook-associated protein FlgL -
  WKI52_RS02320 (WKI52_02320) flgK 451218..452735 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  WKI52_RS02325 (WKI52_02325) - 452751..453233 (-) 483 WP_022553865.1 flagellar protein FlgN -
  WKI52_RS02330 (WKI52_02330) flgM 453248..453514 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  WKI52_RS02335 (WKI52_02335) - 453584..454003 (-) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  WKI52_RS02340 (WKI52_02340) comFC 454077..454766 (-) 690 WP_199803302.1 phosphoribosyltransferase family protein Machinery gene
  WKI52_RS02345 (WKI52_02345) - 454772..455056 (-) 285 WP_022553866.1 competence protein ComFB -
  WKI52_RS02350 (WKI52_02350) comFA 455114..456499 (-) 1386 WP_063636495.1 DEAD/DEAH box helicase Machinery gene
  WKI52_RS02355 (WKI52_02355) - 456607..457455 (-) 849 WP_003151387.1 DegV family protein -
  WKI52_RS02360 (WKI52_02360) degU 457553..458242 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  WKI52_RS02365 (WKI52_02365) degS 458319..459482 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  WKI52_RS02370 (WKI52_02370) - 459705..460352 (+) 648 WP_060657503.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52663.08 Da        Isoelectric Point: 10.2804

>NTDB_id=960180 WKI52_RS02350 WP_063636495.1 455114..456499(-) (comFA) [Bacillus velezensis strain MJ06]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQTPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLNKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQLQLPVFLFVPSVHVLKKTTDYFRKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=960180 WKI52_RS02350 WP_063636495.1 455114..456499(-) (comFA) [Bacillus velezensis strain MJ06]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
CTCAGACCAGCGTATCAAGCGTTTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAACCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTTTTAGTATGGGCGGTTTGCGGATCAGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTTTGTGTCGCAACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAATAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTCCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCGGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(330-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623