Detailed information NTG0960013

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WHL95_RS02035
• Genome accession: NZ_CP149578
• Coordinates: 437799..438947 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa isolate FELIX_MS413
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 432799..443947

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHL95_RS02010	-	433950..434246 (-)	297	WP_031655952.1	DUF167 domain-containing protein	-
WHL95_RS02015	-	434243..434836 (-)	594	WP_003084544.1	YggT family protein	-
WHL95_RS02020	proC	434847..435668 (-)	822	WP_003114890.1	pyrroline-5-carboxylate reductase	-
WHL95_RS02025	-	435680..436372 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
WHL95_RS02030	pilT	436587..437621 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
WHL95_RS02035	pilU	437799..438947 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
WHL95_RS02040	czcD	438955..439854 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
WHL95_RS02045	-	440008..440412 (+)	405	WP_003084560.1	NINE protein	-
WHL95_RS02050	-	440655..442028 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
WHL95_RS02055	-	442025..443209 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=960013 WHL95_RS02035 WP_003100959.1 437799..438947(+) (pilU) [Pseudomonas aeruginosa isolate FELIX_MS413]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=960013 WHL95_RS02035 WP_003100959.1 437799..438947(+) (pilU) [Pseudomonas aeruginosa isolate FELIX_MS413]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382