Detailed information NTG0958882

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACI5EZ_RS04455
• Genome accession: NZ_CP173428
• Coordinates: 916957..918075 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347035.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain CRAB_1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 911957..923075

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI5EZ_RS04425	-	912786..913634 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
ACI5EZ_RS04430	-	913887..914342 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ACI5EZ_RS04435	-	914495..915562 (+)	1068	WP_005120426.1	hypothetical protein	-
ACI5EZ_RS04440	-	915562..915885 (+)	324	WP_000442585.1	RnfH family protein	-
ACI5EZ_RS04445	-	915940..916338 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACI5EZ_RS04450	fur	916450..916887 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACI5EZ_RS04455	pilU	916957..918075 (-)	1119	WP_000347035.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACI5EZ_RS04460	pilT	918103..919140 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACI5EZ_RS04465	-	919267..919959 (+)	693	WP_001108510.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41821.98 Da
• Isoelectric Point: 6.5675

>NTDB_id=958882 ACI5EZ_RS04455 WP_000347035.1 916957..918075(-) (pilU) [Acinetobacter baumannii strain CRAB_1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide

• Length: 1119 bp

>NTDB_id=958882 ACI5EZ_RS04455 WP_000347035.1 916957..918075(-) (pilU) [Acinetobacter baumannii strain CRAB_1]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.817	100	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39