Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACIO04_RS08610 Genome accession   NZ_CP173229
Coordinates   1760100..1760642 (+) Length   180 a.a.
NCBI ID   WP_410679751.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1755100..1765642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO04_RS08585 (ACIO04_08585) - 1755577..1756506 (+) 930 WP_410679750.1 phosphatidate cytidylyltransferase -
  ACIO04_RS08590 (ACIO04_08590) mog 1756525..1757103 (+) 579 WP_264256079.1 molybdopterin adenylyltransferase -
  ACIO04_RS08595 (ACIO04_08595) glnB 1757132..1757470 (+) 339 WP_017805299.1 nitrogen regulatory protein P-II -
  ACIO04_RS08600 (ACIO04_08600) - 1757473..1758525 (+) 1053 WP_264256080.1 AI-2E family transporter -
  ACIO04_RS08605 (ACIO04_08605) - 1758897..1760066 (+) 1170 WP_264256081.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACIO04_RS08610 (ACIO04_08610) pilF/pilF2 1760100..1760642 (+) 543 WP_410679751.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIO04_RS08615 (ACIO04_08615) dprA 1760787..1761887 (+) 1101 WP_410679752.1 DNA-processing protein DprA Machinery gene
  ACIO04_RS08620 (ACIO04_08620) rsmD 1761884..1762492 (-) 609 WP_410679753.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACIO04_RS08625 (ACIO04_08625) pilA 1762648..1764141 (+) 1494 WP_410679754.1 signal recognition particle-docking protein FtsY Machinery gene
  ACIO04_RS08630 (ACIO04_08630) - 1764338..1764544 (-) 207 WP_410679755.1 hypothetical protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20701.70 Da        Isoelectric Point: 8.0752

>NTDB_id=957461 ACIO04_RS08610 WP_410679751.1 1760100..1760642(+) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-1]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENTRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLDKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=957461 ACIO04_RS08610 WP_410679751.1 1760100..1760642(+) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-1]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAACTTGGCATTGGCTATCTTGAGCAAAATGAACTTGGTCTGG
CGAAGCAAAATTTAGACAAAGCTTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCATTGGCATATTTTTATCAG
CTTCAAGGGGAGAATACTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCGGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGATAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.153

98.333

0.483
  pilF Glaesserella parasuis strain SC1401

42.857

100

0.433