Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACH6CW_RS29165 Genome accession   NZ_CP172306
Coordinates   6239990..6240613 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain pat0103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6234990..6245613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH6CW_RS29145 aroB 6235585..6236691 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  ACH6CW_RS29150 aroK 6236741..6237259 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACH6CW_RS29155 pilQ 6237271..6239415 (-) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  ACH6CW_RS29160 comL/pilP 6239469..6239993 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ACH6CW_RS29165 pilO 6239990..6240613 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACH6CW_RS29170 pilN 6240610..6241206 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACH6CW_RS29175 pilM 6241206..6242270 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACH6CW_RS29180 - 6242455..6244926 (+) 2472 WP_260550825.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=951568 ACH6CW_RS29165 WP_003103268.1 6239990..6240613(-) (pilO) [Pseudomonas aeruginosa strain pat0103]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=951568 ACH6CW_RS29165 WP_003103268.1 6239990..6240613(-) (pilO) [Pseudomonas aeruginosa strain pat0103]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401