Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   WHO69_RS13310 Genome accession   NZ_CP148108
Coordinates   2899089..2899682 (-) Length   197 a.a.
NCBI ID   WP_020334615.1    Uniprot ID   A0AAN1CWF1
Organism   Vibrio natriegens isolate FELIX_MS472     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2894089..2904682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO69_RS13290 aroB 2894974..2896074 (-) 1101 WP_020334612.1 3-dehydroquinate synthase -
  WHO69_RS13295 aroK 2896106..2896624 (-) 519 WP_014233147.1 shikimate kinase AroK -
  WHO69_RS13300 pilQ 2896813..2898552 (-) 1740 WP_024372659.1 type IV pilus secretin PilQ family protein Machinery gene
  WHO69_RS13305 pilP 2898584..2899099 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  WHO69_RS13310 pilO 2899089..2899682 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  WHO69_RS13315 pilN 2899675..2900250 (-) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  WHO69_RS13320 pilM 2900234..2901250 (-) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene
  WHO69_RS13325 - 2901394..2903961 (+) 2568 WP_020334618.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23102.59 Da        Isoelectric Point: 4.7130

>NTDB_id=950698 WHO69_RS13310 WP_020334615.1 2899089..2899682(-) (pilO) [Vibrio natriegens isolate FELIX_MS472]
MVNWQDLEMDEIAEWPLIPQLLVISLLILLLQGFGYWLYLVPKEERLNDLIQQEETLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSDHFLYQLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRAHTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=950698 WHO69_RS13310 WP_020334615.1 2899089..2899682(-) (pilO) [Vibrio natriegens isolate FELIX_MS472]
ATGGTTAATTGGCAAGATCTGGAAATGGATGAGATTGCTGAGTGGCCTTTAATCCCTCAGTTGCTAGTCATTAGTTTGTT
GATCCTGTTGCTCCAAGGTTTCGGCTATTGGCTGTACTTGGTGCCTAAGGAAGAGCGATTAAATGACCTCATCCAGCAGG
AAGAAACACTCAAAGTTGCGTTACGAATTAAAGCCAACAAGGTTGCCGCATTACCGCAGCTCCAATCTCAGTTAGATGAA
TTGACTACACGTTACGAGTTGTTGTTGCAGCAATTACCCGCCCAGAAAGAGTTAGCCACCATGCTGGCGTCCGTTAATGA
GCTCGGCATAGACAATAAACTGACCTTCACCCGAATTGACTGGGGAGAAAAGCAGTCTGATCACTTTTTGTATCAGCTGC
CACTTAACATCGAGTTAACCGGTGATTTTCATGACATCGGCCGTTTCTCACAAGCGATTGCAACTTTACCACGCATCATC
ACTTTTCAAGATGTGACTTGGCAACGCGTCAGTCAGGAGAGTGAAACGCTGCACTTCCGGGTAAGAGCTCATACCTATCA
GTTCAAGCCAGAGGAAAAGAAAGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

88.947

96.447

0.858
  pilO Vibrio cholerae strain A1552

70.558

100

0.706