Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   WHO69_RS13315 Genome accession   NZ_CP148108
Coordinates   2899675..2900250 (-) Length   191 a.a.
NCBI ID   WP_024372660.1    Uniprot ID   -
Organism   Vibrio natriegens isolate FELIX_MS472     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2894675..2905250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO69_RS13290 aroB 2894974..2896074 (-) 1101 WP_020334612.1 3-dehydroquinate synthase -
  WHO69_RS13295 aroK 2896106..2896624 (-) 519 WP_014233147.1 shikimate kinase AroK -
  WHO69_RS13300 pilQ 2896813..2898552 (-) 1740 WP_024372659.1 type IV pilus secretin PilQ family protein Machinery gene
  WHO69_RS13305 pilP 2898584..2899099 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  WHO69_RS13310 pilO 2899089..2899682 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  WHO69_RS13315 pilN 2899675..2900250 (-) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  WHO69_RS13320 pilM 2900234..2901250 (-) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene
  WHO69_RS13325 - 2901394..2903961 (+) 2568 WP_020334618.1 PBP1A family penicillin-binding protein -
  WHO69_RS13330 oxyR 2904025..2904933 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22349.65 Da        Isoelectric Point: 5.8969

>NTDB_id=950699 WHO69_RS13315 WP_024372660.1 2899675..2900250(-) (pilN) [Vibrio natriegens isolate FELIX_MS472]
MLHRINLLPWRENQREEHRRRFVGMFILGLFCAVGIQWGIGQFYEIQQDQQQDRLDYLTHYITELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEIAGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKERFGKEFQTFKVSFIFKAVEQEEGGQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=950699 WHO69_RS13315 WP_024372660.1 2899675..2900250(-) (pilN) [Vibrio natriegens isolate FELIX_MS472]
GTGCTACATCGAATTAACTTATTGCCCTGGAGGGAGAACCAAAGGGAAGAACATCGCCGTCGTTTTGTTGGCATGTTCAT
TCTGGGCTTGTTTTGTGCTGTTGGTATTCAATGGGGGATTGGGCAGTTTTATGAGATCCAACAAGATCAGCAGCAAGACC
GACTCGACTATCTTACTCACTATATTACGGAACTTGATCAACGTATTGAAGCAATGAAAATTGCCGAGCAAGAGCACAGC
AAAATATTAGAGCGTTTGAAGGTGGTCGAAGGCTTGCAAAATGGTCGCAACAAAACCACAGAGTTCATGAACCTGATGCC
TGCTGTTATTCCTGAAGGCGTTTACGTTGATAAGATCAAAATGAATGATCTGGAAATCGAAATCGCGGGGATTTCTGATA
GCACACCACGTCTGGCAACCATGCTCGATAACATGGAGCGTTCGGCAAAACTATTGGACGTTGAAATGCACTCTATTGTC
CATGGCAAAGAACGTTTCGGTAAAGAATTTCAGACCTTCAAAGTGTCTTTCATATTTAAAGCGGTAGAGCAGGAAGAGGG
AGGTCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

81.152

100

0.812
  pilN Vibrio cholerae strain A1552

59.896

100

0.602