Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   WHO69_RS13320 Genome accession   NZ_CP148108
Coordinates   2900234..2901250 (-) Length   338 a.a.
NCBI ID   WP_020334617.1    Uniprot ID   A0AAN0Y4M4
Organism   Vibrio natriegens isolate FELIX_MS472     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2895234..2906250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO69_RS13295 aroK 2896106..2896624 (-) 519 WP_014233147.1 shikimate kinase AroK -
  WHO69_RS13300 pilQ 2896813..2898552 (-) 1740 WP_024372659.1 type IV pilus secretin PilQ family protein Machinery gene
  WHO69_RS13305 pilP 2898584..2899099 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  WHO69_RS13310 pilO 2899089..2899682 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  WHO69_RS13315 pilN 2899675..2900250 (-) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  WHO69_RS13320 pilM 2900234..2901250 (-) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene
  WHO69_RS13325 - 2901394..2903961 (+) 2568 WP_020334618.1 PBP1A family penicillin-binding protein -
  WHO69_RS13330 oxyR 2904025..2904933 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -
  WHO69_RS13335 - 2905092..2905820 (+) 729 WP_020334619.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37587.78 Da        Isoelectric Point: 7.2098

>NTDB_id=950700 WHO69_RS13320 WP_020334617.1 2900234..2901250(-) (pilM) [Vibrio natriegens isolate FELIX_MS472]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKSQYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIQCSVQESPLSGEKQAKTSQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAVECELLNPLGLFEMNVAKRKRRAVEWQNFSTAA
GLAVRGIQWLGGHRATSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=950700 WHO69_RS13320 WP_020334617.1 2900234..2901250(-) (pilM) [Vibrio natriegens isolate FELIX_MS472]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCAGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGGCCAGTTA
GCTACCAAGTTTTTGCAACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTC
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAGTCAGTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCAGTGCTCTGTGCAGGAAAGTCCTCTGTCCGGAGAAAAGCAAGCCAAAACGAGTCAATTTAAC
CGTCAGATGGTCGATCGTGTCAAACGTCAGATACAGCTTTATACGTCGATTAATGGGTCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGTTGGCTGAGGAGTTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ATCCTTTAGGCCTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGCGGTGGAATGGCAGAATTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTGGGAGGTCATCGTGCTACATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

74.556

100

0.746
  pilM Vibrio cholerae strain A1552

48.521

100

0.485