Detailed information NTG0950162

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WDR08_RS09990
• Genome accession: NZ_CP148035
• Coordinates: 2047420..2048568 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain S1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2042420..2053568

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WDR08_RS09970 (WDR08_09970)	-	2043157..2044341 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
WDR08_RS09975 (WDR08_09975)	-	2044338..2045711 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
WDR08_RS09980 (WDR08_09980)	-	2045954..2046358 (-)	405	WP_003084560.1	NINE protein	-
WDR08_RS09985 (WDR08_09985)	czcD	2046513..2047412 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
WDR08_RS09990 (WDR08_09990)	pilU	2047420..2048568 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
WDR08_RS09995 (WDR08_09995)	pilT	2048746..2049780 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
WDR08_RS10000 (WDR08_10000)	-	2049995..2050687 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
WDR08_RS10005 (WDR08_10005)	proC	2050699..2051520 (+)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
WDR08_RS10010 (WDR08_10010)	-	2051531..2052124 (+)	594	WP_003084544.1	YggT family protein	-
WDR08_RS10015 (WDR08_10015)	-	2052121..2052417 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=950162 WDR08_RS09990 WP_003100959.1 2047420..2048568(-) (pilU) [Pseudomonas aeruginosa strain S1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=950162 WDR08_RS09990 WP_003100959.1 2047420..2048568(-) (pilU) [Pseudomonas aeruginosa strain S1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382