Detailed information NTG0094967

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: AAA982_RS01520
• Genome accession: NZ_AP028601
• Coordinates: 270945..271943 (+)
• Length: 332 a.a.
• NCBI ID: WP_001809468.1
• Uniprot ID: Q8CWU7
• Organism: Streptococcus pneumoniae strain PF1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 265945..276943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAA982_RS01495 (TKY113292_02870)	leuS	266035..268536 (+)	2502	WP_317649232.1	leucine--tRNA ligase	-
AAA982_RS01500 (TKY113292_02880)	-	268836..269069 (+)	234	Protein_292	GNAT family N-acetyltransferase	-
AAA982_RS01505 (TKY113292_02890)	-	269172..269634 (+)	463	Protein_293	GNAT family protein	-
AAA982_RS01510 (TKY113292_02900)	-	269646..270062 (+)	417	WP_050212581.1	GNAT family N-acetyltransferase	-
AAA982_RS01515	-	270720..270811 (-)	92	Protein_295	type I toxin-antitoxin system Fst family toxin	-
AAA982_RS01520 (TKY113292_02910)	ruvB	270945..271943 (+)	999	WP_001809468.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
AAA982_RS01525 (TKY113292_02920)	-	271924..272496 (+)	573	Protein_297	nucleotidyltransferase family protein	-
AAA982_RS01530 (TKY113292_02930)	-	272727..273485 (+)	759	WP_000466719.1	isoprenyl transferase	-
AAA982_RS01535 (TKY113292_02940)	-	273494..274297 (+)	804	WP_000189858.1	phosphatidate cytidylyltransferase	-
AAA982_RS01540 (TKY113292_02950)	eeP	274319..275578 (+)	1260	WP_000900660.1	RIP metalloprotease RseP	Regulator

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37288.73 Da
• Isoelectric Point: 4.5053

>NTDB_id=94967 AAA982_RS01520 WP_001809468.1 270945..271943(+) (ruvB) [Streptococcus pneumoniae strain PF1]
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Nucleotide

• Length: 999 bp

>NTDB_id=94967 AAA982_RS01520 WP_001809468.1 270945..271943(+) (ruvB) [Streptococcus pneumoniae strain PF1]
ATGAGTAGAATTTTAGATAATGAGATAATGGGGGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATCGGACAGGATAAGGTCAAGGACCAGCTACAAATCTTTATTGAAGCTGCCAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGGCCTCCAGGGCTTGGGAAAACAACCATGGCTTTTGTCATTGCCAATGAACTGGGAGTCAATCTC
AAGCAAACGTCTGGTCCTGTTATCGAAAAAGCGGGTGATTTGGTAGCGATTTTGAATGAGCTAGAGCCTGGGGATGTCCT
TTTTATTGATGAGATCCATCGTTTGCCAATGTCAGTGGAAGAGGTGCTTTATAGTGCTATGGAGGACTTCTACATAGATA
TTATGATTGGGGCTGGTGAGGGTAGTCGTAGTGTTCATTTGGAGTTACCACCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCCAATCCGCTACGGGCACGTTTTGGGATTACAGGCCATATGGAGTATTATGCCCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACTCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGAACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTAATTGAT
GATATTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
TACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTCAATATTGCCGAGGAGCGTGAGACTG
TAGAAGACATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: Q8CWU7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	100	100	1
ruvB	Streptococcus pneumoniae D39	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	99.096	100	0.991
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.429	98.193	0.593
ruvB	Helicobacter pylori 26695	50.774	97.289	0.494
ruvB	Synechocystis sp. PCC 6803	49.84	94.277	0.47