Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   WFA81_RS03030 Genome accession   NZ_CP147890
Coordinates   613999..615363 (+) Length   454 a.a.
NCBI ID   WP_012491916.1    Uniprot ID   -
Organism   Lacticaseibacillus paracasei strain CUDS0725     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 608999..620363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WFA81_RS03005 - 609368..610714 (-) 1347 WP_003576225.1 C1 family peptidase -
  WFA81_RS03010 - 610871..612190 (-) 1320 WP_012491917.1 C1 family peptidase -
  WFA81_RS03015 - 612435..612812 (+) 378 WP_003567260.1 DUF805 domain-containing protein -
  WFA81_RS03020 - 612929..613219 (-) 291 WP_003567258.1 GNAT family N-acetyltransferase -
  WFA81_RS03025 - 613440..613982 (+) 543 WP_003567256.1 dUTP diphosphatase -
  WFA81_RS03030 radA 613999..615363 (+) 1365 WP_012491916.1 DNA repair protein RadA Machinery gene
  WFA81_RS03035 - 615385..616500 (+) 1116 WP_003567252.1 PIN domain-containing protein -
  WFA81_RS03040 - 616603..617499 (-) 897 WP_003567250.1 Rgg/GadR/MutR family transcriptional regulator -
  WFA81_RS03045 - 617683..618570 (+) 888 WP_012491915.1 ABC transporter ATP-binding protein -
  WFA81_RS03050 - 618588..619364 (+) 777 WP_012491914.1 ABC transporter permease subunit -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49127.46 Da        Isoelectric Point: 7.1396

>NTDB_id=949281 WFA81_RS03030 WP_012491916.1 613999..615363(+) (radA) [Lacticaseibacillus paracasei strain CUDS0725]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTAAGSKVKPTRMNDVTITKETRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLADTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=949281 WFA81_RS03030 WP_012491916.1 613999..615363(+) (radA) [Lacticaseibacillus paracasei strain CUDS0725]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGTCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACAGCAGCCGGCAGTAAGG
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGAAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTACTACTGCA
GGTGTCAGGTCAATTGGCCGATACTGGCGGAACTGTTTTGTATGTCTCTGGTGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGCGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTACGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGCGAAGTGACGGCAGAACTGATGCGCATTGCCAAGTCCAAAGGCGTGACCATTTTCATCGTTGGCC
ATGTCACCAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTTGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGACCAATCTTAGTCGAGATTCAAGCCTTGATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGAGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGGCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAATTGGGTTTCAAACGGATTTTTGTACCACGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.212

99.78

0.681
  radA Streptococcus pneumoniae Rx1

68.212

99.78

0.681
  radA Streptococcus pneumoniae D39

68.212

99.78

0.681
  radA Streptococcus pneumoniae R6

68.212

99.78

0.681
  radA Streptococcus pneumoniae TIGR4

68.212

99.78

0.681
  radA Streptococcus mitis SK321

67.991

99.78

0.678
  radA Bacillus subtilis subsp. subtilis str. 168

64.758

100

0.648