Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WDM00_RS11785 Genome accession   NZ_CP147726
Coordinates   2585947..2587053 (-) Length   368 a.a.
NCBI ID   WP_095471735.1    Uniprot ID   -
Organism   Vibrio cholerae strain CNCTC 6536     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2580947..2592053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDM00_RS11760 (WDM00_11770) gshB 2581547..2582503 (+) 957 WP_000593313.1 glutathione synthase -
  WDM00_RS11765 (WDM00_11775) - 2582539..2583102 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  WDM00_RS11770 (WDM00_11780) ruvX 2583183..2583605 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  WDM00_RS11775 (WDM00_11785) tyrS 2583643..2584923 (-) 1281 WP_367007783.1 tyrosine--tRNA ligase -
  WDM00_RS11780 (WDM00_11790) - 2585032..2585829 (-) 798 WP_002051047.1 helix-turn-helix transcriptional regulator -
  WDM00_RS11785 (WDM00_11795) pilU 2585947..2587053 (-) 1107 WP_095471735.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WDM00_RS11790 (WDM00_11800) pilT 2587068..2588105 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  WDM00_RS11795 (WDM00_11805) - 2588131..2588841 (+) 711 WP_248387455.1 YggS family pyridoxal phosphate-dependent enzyme -
  WDM00_RS11800 (WDM00_11810) proC 2588894..2589712 (+) 819 WP_000437946.1 pyrroline-5-carboxylate reductase -
  WDM00_RS11805 (WDM00_11815) - 2589766..2590323 (+) 558 WP_001087261.1 YggT family protein -
  WDM00_RS11810 (WDM00_11820) yggU 2590323..2590613 (+) 291 WP_000741918.1 DUF167 family protein YggU -
  WDM00_RS11815 (WDM00_11825) - 2590659..2591090 (+) 432 WP_001233674.1 DUF4426 domain-containing protein -
  WDM00_RS11820 (WDM00_11830) - 2591160..2591762 (+) 603 WP_000725016.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41321.30 Da        Isoelectric Point: 6.5076

>NTDB_id=948248 WDM00_RS11785 WP_095471735.1 2585947..2587053(-) (pilU) [Vibrio cholerae strain CNCTC 6536]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=948248 WDM00_RS11785 WP_095471735.1 2585947..2587053(-) (pilU) [Vibrio cholerae strain CNCTC 6536]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAGGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGCTATGAAATCGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACAGGGCATTTGTGTATGGCCACGCTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTAGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGAGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.728

100

0.997
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361