Detailed information NTG0948006

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IG620_RS08105
• Genome accession: NZ_CP147682
• Coordinates: 1705745..1706941 (-)
• Length: 398 a.a.
• NCBI ID: WP_005251952.1
• Uniprot ID: N9PSE4
• Organism: Acinetobacter lwoffii strain CCTCC M20191004
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1700745..1711941

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IG620_RS08090 (IG620_08090)	-	1702762..1703790 (+)	1029	WP_005246182.1	AraC family transcriptional regulator	-
IG620_RS08095 (IG620_08095)	gcvH	1703873..1704247 (+)	375	WP_338839256.1	glycine cleavage system protein GcvH	-
IG620_RS08100 (IG620_08100)	-	1704487..1705662 (+)	1176	WP_114541204.1	MFS transporter	-
IG620_RS08105 (IG620_08105)	pilU	1705745..1706941 (-)	1197	WP_005251952.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IG620_RS08110 (IG620_08110)	-	1707503..1708066 (-)	564	WP_004734498.1	tetratricopeptide repeat protein	-
IG620_RS08115 (IG620_08115)	-	1708270..1709604 (+)	1335	WP_338839257.1	ISNCY family transposase	-
IG620_RS08120 (IG620_08120)	-	1710223..1711272 (-)	1050	WP_338839258.1	3-deoxy-7-phosphoheptulonate synthase	-

Sequence

Protein

• Length: 398 a.a.
• Molecular weight: 45112.72 Da
• Isoelectric Point: 6.6224

>NTDB_id=948006 IG620_RS08105 WP_005251952.1 1705745..1706941(-) (pilU) [Acinetobacter lwoffii strain CCTCC M20191004]
MFTAELLDEAKKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNDKQRKEFETELEC
NFAITVPGVSRFRVNVFVQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLMSDLILKGEFHELKAIMAKS
RELGMQTFDQALFDLYNQGEISYEEALRNADSTNELRLQIKLKSNRQDTTVATEATQFTMMQEPKAEEKQAENTSVSE

Nucleotide

• Length: 1197 bp

>NTDB_id=948006 IG620_RS08105 WP_005251952.1 1705745..1706941(-) (pilU) [Acinetobacter lwoffii strain CCTCC M20191004]
ATGTTCACGGCAGAATTGTTGGACGAAGCAAAAAAATTCATGCATCACATGCTGACTAAAGTGGTGGAATATGGCGGTTC
AGATTTATTTATTACGGCTGATTTTCCACCGAGTATTAAACATCAAGGCTTAATGAAACCTTTTGGACAGCAGGAACTCA
CAGCTGAAAAAACCAAGCTGTTTGCCTACAGTCTGATGAATGATAAACAGCGTAAAGAATTTGAAACTGAACTTGAGTGC
AATTTTGCCATCACCGTGCCAGGTGTTTCCCGTTTTCGGGTCAATGTGTTTGTACAACAACTTAATGTTGGCATGGTGAT
TCGTACCATTACTGCAGAAATTCCAAATTTCCAGAAGCTGAAATTACCGGAATCTCTGAAACATGTGATTATGGAAAAAC
GTGGACTGGTATTGGTAGTCGGAGGAACAGGTTCCGGTAAATCAACTTCACTCGCAGCCATGATTGACTATCGAAATGAA
AATTCAGCCGGGCATATCATTACCGTAGAAGATCCGGTTGAATATGTGCACAAGCATAAAAAATCCATGATTACTCATCG
TGAAGTCGGAGTGGACTGCCATTCCTGGCATAATGCGCTGAAAAATACCTTGCGTCAGGCACCAGATGTGATTCTGATCG
GCGAAATTCGCGATACTGAAACCATGGAACACGCGATTGCTTTTGCTGAAACTGGACATCTGTGTCTGGGAACGCTGCAC
TCGAACAATGCCAACCAGACTTTGGACCGGATTATCAACTTCTTCCCGGAAGAACGCCGGAACCAGCTATTAATGGATTT
GTCATCCAATATGAAGGCGATCATTTCACAGCGTCTGATTCGTACTCAAGATGGTAAAGGCCGTCGTGCAGCGATTGAAA
TCATGTTAAATACGCCACTGATGTCTGACCTGATTTTGAAAGGTGAATTCCACGAATTGAAAGCGATTATGGCGAAATCG
CGTGAACTGGGCATGCAGACCTTTGACCAGGCCTTATTTGACCTGTATAACCAGGGTGAAATTTCTTATGAAGAAGCCTT
GCGCAATGCCGATTCGACCAATGAACTGCGCTTACAAATCAAGCTGAAAAGTAACCGACAGGACACTACCGTAGCCACAG
AGGCAACCCAATTCACCATGATGCAAGAGCCAAAAGCGGAAGAGAAACAAGCAGAGAATACTTCGGTGTCAGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-282)

3D structure

• AlphaFold DB: N9PSE4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.494	87.437	0.538
pilU	Acinetobacter baylyi ADP1	55.908	87.186	0.487
pilU	Vibrio cholerae strain A1552	52.394	89.196	0.467
pilT	Pseudomonas aeruginosa PAK	44.172	81.91	0.362