Detailed information NTG0947581

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WCU24_RS01995
• Genome accession: NZ_CP147619
• Coordinates: 428114..429262 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain GN05356
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 423114..434262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WCU24_RS01970 (WCU24_01970)	-	424265..424561 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
WCU24_RS01975 (WCU24_01975)	-	424558..425151 (-)	594	WP_003084544.1	YggT family protein	-
WCU24_RS01980 (WCU24_01980)	proC	425162..425983 (-)	822	WP_338852233.1	pyrroline-5-carboxylate reductase	-
WCU24_RS01985 (WCU24_01985)	-	425995..426687 (-)	693	WP_003459261.1	YggS family pyridoxal phosphate-dependent enzyme	-
WCU24_RS01990 (WCU24_01990)	pilT	426902..427936 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
WCU24_RS01995 (WCU24_01995)	pilU	428114..429262 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
WCU24_RS02000 (WCU24_02000)	czcD	429270..430169 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
WCU24_RS02005 (WCU24_02005)	-	430323..430727 (+)	405	WP_003084560.1	NINE protein	-
WCU24_RS02010 (WCU24_02010)	-	430970..432343 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
WCU24_RS02015 (WCU24_02015)	-	432340..433524 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=947581 WCU24_RS01995 WP_003100959.1 428114..429262(+) (pilU) [Pseudomonas aeruginosa strain GN05356]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=947581 WCU24_RS01995 WP_003100959.1 428114..429262(+) (pilU) [Pseudomonas aeruginosa strain GN05356]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382