Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WBI56_RS12905 Genome accession   NZ_CP147492
Coordinates   2504868..2505485 (-) Length   205 a.a.
NCBI ID   WP_338730593.1    Uniprot ID   -
Organism   Bacillus subtilis strain MC4-2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2499868..2510485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBI56_RS12875 (WBI56_12875) rpsT 2500170..2500436 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  WBI56_RS12880 (WBI56_12880) holA 2500451..2501494 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  WBI56_RS12885 (WBI56_12885) - 2501534..2501683 (-) 150 WP_003229985.1 hypothetical protein -
  WBI56_RS12890 (WBI56_12890) yqzM 2501724..2501858 (+) 135 WP_003229983.1 YqzM family protein -
  WBI56_RS12895 (WBI56_12895) comEC 2501898..2504228 (-) 2331 WP_338730592.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WBI56_RS12900 (WBI56_12900) comEB 2504232..2504801 (-) 570 WP_003229978.1 ComE operon protein 2 -
  WBI56_RS12905 (WBI56_12905) comEA 2504868..2505485 (-) 618 WP_338730593.1 competence protein ComEA Machinery gene
  WBI56_RS12910 (WBI56_12910) comER 2505569..2506390 (+) 822 WP_338730594.1 late competence protein ComER -
  WBI56_RS12915 (WBI56_12915) yqeM 2506456..2507199 (-) 744 WP_151870165.1 class I SAM-dependent methyltransferase -
  WBI56_RS12920 (WBI56_12920) rsfS 2507196..2507552 (-) 357 WP_014480315.1 ribosome silencing factor -
  WBI56_RS12925 (WBI56_12925) yqeK 2507570..2508130 (-) 561 WP_017696284.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WBI56_RS12930 (WBI56_12930) nadD 2508120..2508689 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  WBI56_RS12935 (WBI56_12935) yhbY 2508701..2508991 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  WBI56_RS12940 (WBI56_12940) aroE 2508985..2509827 (-) 843 WP_338730595.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21812.61 Da        Isoelectric Point: 4.7220

>NTDB_id=946356 WBI56_RS12905 WP_338730593.1 2504868..2505485(-) (comEA) [Bacillus subtilis strain MC4-2]
MNWLSQHKKAIILAAFAAVFTVIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNEIIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=946356 WBI56_RS12905 WP_338730593.1 2504868..2505485(-) (comEA) [Bacillus subtilis strain MC4-2]
ATGAATTGGTTGAGTCAGCATAAGAAAGCAATTATTTTAGCGGCTTTTGCGGCTGTTTTCACAGTGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAATAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAGGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

36.406

100

0.385
  comEA Staphylococcus aureus N315

35.484

100

0.376