Detailed information NTG0945701

Bioinformatically predicted

Overview

• Name: stkP
• Type: Regulator
• Locus tag: ACF0HW_RS10500
• Genome accession: NZ_CP171208
• Coordinates: 2146362..2148305 (-)
• Length: 647 a.a.
• NCBI ID: WP_012117534.1
• Uniprot ID: A7Z4J7
• Organism: Bacillus amyloliquefaciens strain HN11
• Function: require for competence development; phosphorylate ComE (predicted from homology) Competence regulation

Genomic Context

Location: 2141362..2153305

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF0HW_RS10470	-	2143038..2143400 (-)	363	WP_003154327.1	Asp23/Gls24 family envelope stress response protein	-
ACF0HW_RS10475	rpmB	2143659..2143847 (+)	189	WP_003154328.1	50S ribosomal protein L28	-
ACF0HW_RS10480	spoVM	2143925..2144005 (-)	81	WP_003154329.1	stage V sporulation protein SpoVM	-
ACF0HW_RS10485	-	2144092..2144736 (-)	645	WP_012117537.1	thiamine diphosphokinase	-
ACF0HW_RS10490	rpe	2144805..2145455 (-)	651	WP_012117536.1	ribulose-phosphate 3-epimerase	-
ACF0HW_RS10495	rsgA	2145457..2146347 (-)	891	WP_012117535.1	ribosome small subunit-dependent GTPase A	-
ACF0HW_RS10500	stkP	2146362..2148305 (-)	1944	WP_012117534.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
ACF0HW_RS10505	-	2148299..2149060 (-)	762	WP_041482191.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
ACF0HW_RS10510	rlmN	2149066..2150157 (-)	1092	WP_003154339.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
ACF0HW_RS10515	rsmB	2150161..2151504 (-)	1344	WP_012117532.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
ACF0HW_RS10520	fmt	2151491..2152444 (-)	954	WP_012117531.1	methionyl-tRNA formyltransferase	-
ACF0HW_RS10525	def	2152449..2152931 (-)	483	WP_012117530.1	peptide deformylase	-

Sequence

Protein

• Length: 647 a.a.
• Molecular weight: 71694.28 Da
• Isoelectric Point: 5.0248

>NTDB_id=945701 ACF0HW_RS10500 WP_012117534.1 2146362..2148305(-) (stkP) [Bacillus amyloliquefaciens strain HN11]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQNPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPEGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide

• Length: 1944 bp

>NTDB_id=945701 ACF0HW_RS10500 WP_012117534.1 2146362..2148305(-) (stkP) [Bacillus amyloliquefaciens strain HN11]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCCGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAAATCCCTTTTGACGGAGAAT
CAGCCGTCAGTATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTTATTACTGACGGCGCAAAAGCCGCGCCTCAGGCGGAAACGCCGGGGGAGACGGATGATAAGGAACAGCAGCCCGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCGGCTGTTCTTGCGGTCAC
CGTGTTTCCGTCTTTATTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTCCGGGCGGATCCTGATATAACGGACATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGACGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAGAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGAACCCGTCAGCCGGCACCGACATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCGGAAGGACAAG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCATTTAAAATAAAAGAGCCGACTGAAAAAACAA
TCGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGATTGA

Domains

Predicted by InterProScan.

• PASTA domain (PASTA) (495-556)

• PASTA domain (PASTA) (431-489)

• PASTA domain (PASTA) (358-419)

• Protein kinase domain (Pkinase) (11-260)

3D structure

• AlphaFold DB: A7Z4J7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
stkP	Streptococcus pneumoniae TIGR4	38.799	95.209	0.369
stkP	Streptococcus pneumoniae D39	38.636	95.209	0.368
stkP	Streptococcus pneumoniae R6	38.636	95.209	0.368
stkP	Streptococcus pneumoniae Rx1	38.474	95.209	0.366
pknB	Streptococcus mutans UA159	40.838	88.563	0.362