Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   ACFXAA_RS03335 Genome accession   NZ_CP170717
Coordinates   749123..751066 (-) Length   647 a.a.
NCBI ID   WP_061861450.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5014     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 744123..756066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAA_RS03305 (ACFXAA_03305) - 745799..746161 (-) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -
  ACFXAA_RS03310 (ACFXAA_03310) rpmB 746420..746608 (+) 189 WP_003154328.1 50S ribosomal protein L28 -
  ACFXAA_RS03315 (ACFXAA_03315) spoVM 746686..746766 (-) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  ACFXAA_RS03320 (ACFXAA_03320) - 746853..747497 (-) 645 WP_061861452.1 thiamine diphosphokinase -
  ACFXAA_RS03325 (ACFXAA_03325) rpe 747566..748216 (-) 651 WP_317859642.1 ribulose-phosphate 3-epimerase -
  ACFXAA_RS03330 (ACFXAA_03330) rsgA 748218..749108 (-) 891 WP_033574582.1 ribosome small subunit-dependent GTPase A -
  ACFXAA_RS03335 (ACFXAA_03335) stkP 749123..751066 (-) 1944 WP_061861450.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ACFXAA_RS03340 (ACFXAA_03340) - 751060..751821 (-) 762 WP_007409750.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ACFXAA_RS03345 (ACFXAA_03345) rlmN 751827..752918 (-) 1092 WP_003154339.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACFXAA_RS03350 (ACFXAA_03350) rsmB 752922..754262 (-) 1341 WP_015417450.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ACFXAA_RS03355 (ACFXAA_03355) fmt 754252..755202 (-) 951 WP_015417449.1 methionyl-tRNA formyltransferase -
  ACFXAA_RS03360 (ACFXAA_03360) def 755207..755689 (-) 483 WP_012117530.1 peptide deformylase -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71722.29 Da        Isoelectric Point: 4.9835

>NTDB_id=944990 ACFXAA_RS03335 WP_061861450.1 749123..751066(-) (stkP) [Bacillus amyloliquefaciens strain JP5014]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQNPSAGTDMDAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPEGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKIIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=944990 ACFXAA_RS03335 WP_061861450.1 749123..751066(-) (stkP) [Bacillus amyloliquefaciens strain JP5014]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCTGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAAGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAAATTCCTTTTGACGGAGAAT
CAGCCGTCAGCATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTCATTACTGACGGCGCAAAAGCCGCGCCTCAGGCGGAAACGCCGGGGGAGACAGATGATAAGGAACAGCAGCCCGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCAGCTGTTCTTGCGGTCAC
CGTGTTCCCGTCTTTGTTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTCCGGGCGGATCCTGATATAACGGATATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGACGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAGAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGAACCCGTCAGCCGGGACTGACATGGACGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCGGAAGGACAAG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCATTTAAAATAAAAGAGCCGACTGAAAAAATAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

39.123

95.209

0.372
  stkP Streptococcus pneumoniae D39

38.961

95.209

0.371
  stkP Streptococcus pneumoniae R6

38.961

95.209

0.371
  stkP Streptococcus pneumoniae Rx1

38.799

95.209

0.369
  pknB Streptococcus mutans UA159

40.838

88.563

0.362