Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   ACFXAD_RS12755 Genome accession   NZ_CP170653
Coordinates   2598249..2600192 (-) Length   647 a.a.
NCBI ID   WP_012117534.1    Uniprot ID   A7Z4J7
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 2593249..2605192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS12725 (ACFXAD_12725) - 2594925..2595287 (-) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -
  ACFXAD_RS12730 (ACFXAD_12730) rpmB 2595546..2595734 (+) 189 WP_003154328.1 50S ribosomal protein L28 -
  ACFXAD_RS12735 (ACFXAD_12735) spoVM 2595812..2595892 (-) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  ACFXAD_RS12740 (ACFXAD_12740) - 2595979..2596623 (-) 645 WP_053573430.1 thiamine diphosphokinase -
  ACFXAD_RS12745 (ACFXAD_12745) rpe 2596692..2597342 (-) 651 WP_014417749.1 ribulose-phosphate 3-epimerase -
  ACFXAD_RS12750 (ACFXAD_12750) rsgA 2597344..2598234 (-) 891 WP_033574582.1 ribosome small subunit-dependent GTPase A -
  ACFXAD_RS12755 (ACFXAD_12755) stkP 2598249..2600192 (-) 1944 WP_012117534.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  ACFXAD_RS12760 (ACFXAD_12760) - 2600186..2600947 (-) 762 WP_007409750.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ACFXAD_RS12765 (ACFXAD_12765) rlmN 2600953..2602044 (-) 1092 WP_053573429.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACFXAD_RS12770 (ACFXAD_12770) rsmB 2602048..2603391 (-) 1344 WP_014417745.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ACFXAD_RS12775 (ACFXAD_12775) fmt 2603378..2604331 (-) 954 WP_014417744.1 methionyl-tRNA formyltransferase -
  ACFXAD_RS12780 (ACFXAD_12780) def 2604336..2604818 (-) 483 WP_014417743.1 peptide deformylase -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71694.28 Da        Isoelectric Point: 5.0248

>NTDB_id=944914 ACFXAD_RS12755 WP_012117534.1 2598249..2600192(-) (stkP) [Bacillus amyloliquefaciens strain JP5011]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDSEAGTVIDQNPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPEGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=944914 ACFXAD_RS12755 WP_012117534.1 2598249..2600192(-) (stkP) [Bacillus amyloliquefaciens strain JP5011]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCCGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGCATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAAATTCCTTTTGACGGAGAAT
CAGCCGTCAGCATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTTATTACTGACGGCGCAAAAGCCGCGCCTCAGGCGGAAACGCCGGGGGAGACGGATGATAAGGAACAGCAGCCCGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCAGCTGTTCTTGCGGTCAC
CGTGTTCCCGTCTTTGTTTATGCCTAAAGATGTAAGCGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTCCGGGCGGATCCTGATATAACGGATATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGGCGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAAAACGATGACAGCGAGGCCGGCACCGTAATTGATCAGAACCCCTCAGCCGGCACCGATATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCGGAAGGACAAG
TCATGAAGCAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCTTTTAAAATAAAAGAGCCGACTGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z4J7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.799

95.209

0.369
  stkP Streptococcus pneumoniae D39

38.636

95.209

0.368
  stkP Streptococcus pneumoniae R6

38.636

95.209

0.368
  stkP Streptococcus pneumoniae Rx1

38.474

95.209

0.366
  pknB Streptococcus mutans UA159

40.838

88.563

0.362