Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   ACFXAD_RS01090 Genome accession   NZ_CP170653
Coordinates   188843..189361 (+) Length   172 a.a.
NCBI ID   WP_004392979.1    Uniprot ID   A7ZAU8
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 183843..194361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS01070 (ACFXAD_01070) - 184921..185127 (+) 207 WP_004392973.1 DUF951 domain-containing protein -
  ACFXAD_RS01075 (ACFXAD_01075) - 185174..187177 (+) 2004 WP_053573836.1 molybdopterin-dependent oxidoreductase -
  ACFXAD_RS01080 (ACFXAD_01080) ychF 187304..188404 (+) 1101 WP_004392975.1 redox-regulated ATPase YchF -
  ACFXAD_RS01085 (ACFXAD_01085) rpsF 188516..188803 (+) 288 WP_004392977.1 30S ribosomal protein S6 -
  ACFXAD_RS01090 (ACFXAD_01090) ssbA 188843..189361 (+) 519 WP_004392979.1 single-stranded DNA-binding protein SsbA Machinery gene
  ACFXAD_RS01095 (ACFXAD_01095) rpsR 189405..189644 (+) 240 WP_004392983.1 30S ribosomal protein S18 -
  ACFXAD_RS01100 (ACFXAD_01100) - 189837..190418 (+) 582 WP_053573837.1 bifunctional transcriptional activator/DNA repair enzyme AdaA -
  ACFXAD_RS01105 (ACFXAD_01105) - 190415..190972 (+) 558 WP_053573838.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ACFXAD_RS01110 (ACFXAD_01110) xth 190969..191727 (+) 759 WP_053573839.1 exodeoxyribonuclease III -
  ACFXAD_RS01115 (ACFXAD_01115) - 191790..192728 (+) 939 WP_053573840.1 LacI family DNA-binding transcriptional regulator -
  ACFXAD_RS01120 (ACFXAD_01120) - 192785..193165 (+) 381 WP_053573841.1 VOC family protein -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 18745.31 Da        Isoelectric Point: 4.7621

>NTDB_id=944829 ACFXAD_RS01090 WP_004392979.1 188843..189361(+) (ssbA) [Bacillus amyloliquefaciens strain JP5011]
MLNRVVLVGRLTKDPELRYTPSGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNSGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=944829 ACFXAD_RS01090 WP_004392979.1 188843..189361(+) (ssbA) [Bacillus amyloliquefaciens strain JP5011]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAGCGGTGCGGCTGTCGC
CACGTTTACTCTTGCTGTGAATCGTACATTCACTAACCAGTCCGGAGAGCGTGAAGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTTCTTAAAAAAGGAAGCCTTGCCGGCGTAGACGGCCGACTCCAGACAAGA
AATTATGAAAACCAGCAGGGACAGCGTGTCTTCGTGACAGAAGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCTAAAAA
CAGCGGCGGTTCTGGATCAGGCGGATACAACGAAGGAAACAGCGGCGGAGGCCAATACTTTGGCGGAGGCCAAAATGATA
ATCCGTTCGGCGGAAATCAGAACAACCAGAGACGAAATCAAGGGAACAGCTTTAATGATGATCCATTTGCCAACGACGGC
AAGCCGATTGACATCTCGGATGATGATCTTCCGTTCTAA

Domains

Predicted by InterProScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZAU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

98.837

100

0.988
  ssb Latilactobacillus sakei subsp. sakei 23K

58.757

100

0.605
  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395
  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378