Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   ACFW99_RS19965 Genome accession   NZ_CP170651
Coordinates   4051934..4052452 (-) Length   172 a.a.
NCBI ID   WP_004392979.1    Uniprot ID   A7ZAU8
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 4046934..4057452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS19935 (ACFW99_19935) - 4048130..4048510 (-) 381 WP_039253120.1 VOC family protein -
  ACFW99_RS19940 (ACFW99_19940) - 4048567..4049505 (-) 939 WP_061861076.1 LacI family DNA-binding transcriptional regulator -
  ACFW99_RS19945 (ACFW99_19945) xth 4049568..4050326 (-) 759 WP_033574264.1 exodeoxyribonuclease III -
  ACFW99_RS19950 (ACFW99_19950) - 4050323..4050880 (-) 558 WP_061861077.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ACFW99_RS19955 (ACFW99_19955) - 4050877..4051458 (-) 582 WP_039253109.1 bifunctional transcriptional activator/DNA repair enzyme AdaA -
  ACFW99_RS19960 (ACFW99_19960) rpsR 4051651..4051890 (-) 240 WP_004392983.1 30S ribosomal protein S18 -
  ACFW99_RS19965 (ACFW99_19965) ssbA 4051934..4052452 (-) 519 WP_004392979.1 single-stranded DNA-binding protein SsbA Machinery gene
  ACFW99_RS19970 (ACFW99_19970) rpsF 4052492..4052779 (-) 288 WP_004392977.1 30S ribosomal protein S6 -
  ACFW99_RS19975 (ACFW99_19975) ychF 4052891..4053991 (-) 1101 WP_004392975.1 redox-regulated ATPase YchF -
  ACFW99_RS19980 (ACFW99_19980) - 4054118..4056121 (-) 2004 WP_015418650.1 molybdopterin-dependent oxidoreductase -
  ACFW99_RS19985 (ACFW99_19985) - 4056168..4056374 (-) 207 WP_004392973.1 DUF951 domain-containing protein -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 18745.31 Da        Isoelectric Point: 4.7621

>NTDB_id=944816 ACFW99_RS19965 WP_004392979.1 4051934..4052452(-) (ssbA) [Bacillus amyloliquefaciens strain JP5025]
MLNRVVLVGRLTKDPELRYTPSGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNSGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=944816 ACFW99_RS19965 WP_004392979.1 4051934..4052452(-) (ssbA) [Bacillus amyloliquefaciens strain JP5025]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAGCGGTGCGGCTGTCGC
CACGTTTACTCTTGCTGTGAATCGTACATTCACGAACCAGTCCGGAGAACGTGAAGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTTCTTAAAAAAGGAAGCCTTGCCGGCGTAGACGGCCGACTCCAGACAAGA
AATTATGAAAACCAGCAGGGACAGCGTGTCTTCGTGACAGAAGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCTAAAAA
CAGCGGCGGTTCTGGATCAGGCGGATACAACGAAGGAAACAGCGGCGGAGGCCAATACTTTGGCGGAGGCCAAAATGATA
ATCCGTTCGGCGGAAATCAGAACAACCAGAGACGAAATCAAGGGAACAGCTTTAATGATGATCCATTTGCCAACGACGGC
AAGCCGATTGACATCTCGGATGATGATCTTCCGTTCTAA

Domains

Predicted by InterProScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZAU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

98.837

100

0.988
  ssb Latilactobacillus sakei subsp. sakei 23K

58.757

100

0.605
  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395
  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378