Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACFI6I_RS26205 Genome accession   NZ_CP170370
Coordinates   5677081..5677704 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PAO1_WT_HKU     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5672081..5682704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFI6I_RS26185 (ACFI6I_26185) aroB 5672677..5673783 (-) 1107 WP_003114574.1 3-dehydroquinate synthase -
  ACFI6I_RS26190 (ACFI6I_26190) aroK 5673833..5674351 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ACFI6I_RS26195 (ACFI6I_26195) pilQ 5674363..5676507 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  ACFI6I_RS26200 (ACFI6I_26200) pilP 5676561..5677084 (-) 524 Protein_5168 type 4a pilus biogenesis lipoprotein PilP -
  ACFI6I_RS26205 (ACFI6I_26205) pilO 5677081..5677704 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFI6I_RS26210 (ACFI6I_26210) pilN 5677701..5678297 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ACFI6I_RS26215 (ACFI6I_26215) pilM 5678297..5679361 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ACFI6I_RS26220 (ACFI6I_26220) - 5679546..5682014 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=942675 ACFI6I_RS26205 WP_003103268.1 5677081..5677704(-) (pilO) [Pseudomonas aeruginosa strain PAO1_WT_HKU]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=942675 ACFI6I_RS26205 WP_003103268.1 5677081..5677704(-) (pilO) [Pseudomonas aeruginosa strain PAO1_WT_HKU]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401