Detailed information NTG0942485

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSGK_RS10915
• Genome accession: NZ_CP146285
• Coordinates: 2434378..2435583 (-)
• Length: 401 a.a.
• NCBI ID: WP_021216620.1
• Uniprot ID: -
• Organism: Pseudomonas solani strain GK13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2429378..2440583

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSGK_RS10895 (PSGK_10810)	-	2429516..2431471 (+)	1956	WP_374981452.1	acetoacetate--CoA ligase	-
PSGK_RS10900 (PSGK_10815)	-	2431629..2432582 (+)	954	WP_052351453.1	DUF4431 domain-containing protein	-
PSGK_RS10905 (PSGK_10820)	-	2432658..2433404 (+)	747	WP_374981453.1	substrate-binding periplasmic protein	-
PSGK_RS10910 (PSGK_10825)	-	2433547..2434221 (+)	675	WP_374981454.1	hypothetical protein	-
PSGK_RS10915 (PSGK_10830)	pilU	2434378..2435583 (-)	1206	WP_021216620.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSGK_RS10920 (PSGK_10835)	-	2435742..2436020 (+)	279	WP_021216621.1	peptidylprolyl isomerase	-
PSGK_RS10925 (PSGK_10840)	-	2436169..2437758 (+)	1590	WP_031286787.1	ABC-F family ATPase	-
PSGK_RS10930 (PSGK_10845)	-	2437821..2439038 (-)	1218	WP_374981455.1	winged helix-turn-helix domain-containing protein	-
PSGK_RS10935 (PSGK_10850)	-	2439061..2439561 (-)	501	WP_374981456.1	DUF3291 domain-containing protein	-
PSGK_RS10940 (PSGK_10855)	-	2439585..2440466 (-)	882	WP_184489397.1	PhzF family phenazine biosynthesis protein	-

Sequence

Protein

• Length: 401 a.a.
• Molecular weight: 44243.49 Da
• Isoelectric Point: 6.6005

>NTDB_id=942485 PSGK_RS10915 WP_021216620.1 2434378..2435583(-) (pilU) [Pseudomonas solani strain GK13]
MLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLGTDPLKPGDVARIAESIMDPEQRGEFDRELEMNLAISLPNIGRFRINI
FKQRNEVSIVARNIKLDIPKFEDLKLPETLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIEDPVEYV
HRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRIINFFP
EERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTSTISDLIKRGDFSSIKEIMEKSKALGMQTFDQALFDLVI
EGSIQEEEAIKNADSANNLRLKLKLHRDGPGTRPTAAAPTPPPAPTPQATPTQDAASWGLELKLEDLPEEDPPPEDPGKH
Y

Nucleotide

• Length: 1206 bp

>NTDB_id=942485 PSGK_RS10915 WP_021216620.1 2434378..2435583(-) (pilU) [Pseudomonas solani strain GK13]
ATGCTCAAGGTTCTGGCCTCCCAGGATGGCTCGGACCTTTACCTTTCCACCGGCGCGCCGCCCTGCGCCAAGTTCAACGG
CGTGCTCAAGCCGCTGGGCACCGACCCGCTCAAGCCGGGCGACGTGGCGCGTATTGCCGAATCCATCATGGACCCGGAGC
AGCGCGGTGAATTCGACCGTGAGCTGGAGATGAACCTGGCCATCTCCCTGCCCAACATCGGGCGGTTCCGTATCAACATC
TTCAAGCAGCGCAACGAGGTTTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCCAAGTTCGAAGACCTGAAGCTGCC
CGAAACCCTGCTCAAGGTGGTAATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCTCGGGCAAGTCCACCT
CCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAAGACCCGGTGGAATACGTG
CACCGGCACAAGAAGTCCATCATCAACCAGCGCGAAGTGGGGGTGGACACCCGCAGCTTCCACGCCGCCCTGAAGAACAC
CCTGCGCCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGTGACCGCGAGACCATGGAGCACGCCCTGGCCTTCGCCG
ACACCGGCCACCTGGCGATCTCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATCATCAACTTCTTCCCC
GAGGAACGCCGCCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCGCCTGGTGCGCACCTC
GGACGGCAAGCGCCGCGCGGCGGTGGAAGTGCTGCTGGGCACCTCCACCATCAGCGACCTGATCAAGCGCGGCGACTTCA
GCTCGATCAAGGAAATCATGGAGAAATCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTGTTCGACCTGGTGATC
GAGGGCTCGATCCAGGAAGAGGAGGCGATCAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAAGCTCAAGCTGCACCG
CGACGGCCCCGGCACCCGGCCTACCGCCGCTGCACCGACCCCGCCTCCCGCACCGACGCCCCAGGCCACGCCGACCCAGG
ATGCCGCCAGCTGGGGCCTGGAACTCAAGCTCGAAGACCTGCCCGAAGAAGATCCGCCGCCGGAAGACCCCGGCAAGCAC
TACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (105-266)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	51.244	100	0.514
pilU	Acinetobacter baylyi ADP1	53.314	86.534	0.461
pilU	Vibrio cholerae strain A1552	51.744	85.786	0.444
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.108	95.012	0.372