Detailed information NTG0942248

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VP398_RS05360
• Genome accession: NZ_CP146231
• Coordinates: 1126172..1127230 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain G20AB08
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1121172..1132230

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP398_RS05335 (VP398_05335)	aroK	1121442..1122011 (-)	570	WP_000643712.1	shikimate kinase AroK	-
VP398_RS05340 (VP398_05340)	pilQ	1122023..1124188 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
VP398_RS05345 (VP398_05345)	pilP	1124256..1124783 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
VP398_RS05350 (VP398_05350)	pilO	1124794..1125534 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
VP398_RS05355 (VP398_05355)	pilN	1125531..1126172 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
VP398_RS05360 (VP398_05360)	pilM	1126172..1127230 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
VP398_RS05365 (VP398_05365)	ponA	1127393..1129948 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
VP398_RS05370 (VP398_05370)	-	1129966..1130790 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
VP398_RS05375 (VP398_05375)	-	1130793..1131125 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=942248 VP398_RS05360 WP_002014066.1 1126172..1127230(-) (pilM) [Acinetobacter baumannii strain G20AB08]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=942248 VP398_RS05360 WP_002014066.1 1126172..1127230(-) (pilM) [Acinetobacter baumannii strain G20AB08]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423