Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   V6667_RS06800 Genome accession   NZ_CP145811
Coordinates   1470956..1472683 (-) Length   575 a.a.
NCBI ID   WP_338809077.1    Uniprot ID   -
Organism   Neisseria leonii strain CCUG 45853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1465956..1477683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6667_RS06770 (V6667_06770) - 1466989..1467303 (-) 315 WP_274584870.1 hypothetical protein -
  V6667_RS06775 (V6667_06775) - 1467355..1467531 (-) 177 WP_274584869.1 DNA gyrase inhibitor YacG -
  V6667_RS06780 (V6667_06780) zapD 1467534..1468295 (-) 762 WP_274584868.1 cell division protein ZapD -
  V6667_RS06785 (V6667_06785) coaE 1468303..1468920 (-) 618 WP_274584867.1 dephospho-CoA kinase -
  V6667_RS06790 (V6667_06790) - 1468917..1469738 (-) 822 WP_338809073.1 A24 family peptidase -
  V6667_RS06795 (V6667_06795) - 1469738..1470949 (-) 1212 WP_338809075.1 type II secretion system F family protein -
  V6667_RS06800 (V6667_06800) pilF 1470956..1472683 (-) 1728 WP_338809077.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  V6667_RS06810 (V6667_06810) asd 1473430..1474551 (+) 1122 WP_338809078.1 aspartate-semialdehyde dehydrogenase -
  V6667_RS06815 (V6667_06815) ilvD 1474696..1476558 (+) 1863 WP_338809080.1 dihydroxy-acid dehydratase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 61983.32 Da        Isoelectric Point: 6.2027

>NTDB_id=940022 V6667_RS06800 WP_338809077.1 1470956..1472683(-) (pilF) [Neisseria leonii strain CCUG 45853]
MNRAVRRQAVCFGSAEPENGLADGGGMASGALRILAGHFGWDGTVLRRCEEWARQGLLLPKVFEEGLCTPDEAAAVLAQA
VGRPYVDLAAFSFASEDTAMTAEQMRRFGCVPLARRQGRLLAGVADPTDPALQRLAFVCAEPVDLAVVRYDLLVQRLAES
GGGTAGILAQMPQEEAGTAAAWAEEAGDGPVARFVQQVLEDAWAAGASDVHFEFYEQAARVRFRVDGVLREVVQPPHAVR
RRLVSRIKVMAGLDIAEQRLPQDGRLQTVLGGRTADLRISTLPTLFGEKVVVRILNGDAQAPDLARLGLEPCQYDLLLAE
IRRPYGMILVTGPTGSGKTLSLYACLNELNSERVNIAAVEDPAEINLAGINQVNVNEKQGLTFAEALRAFLRQDPDVLMV
GEIRDLETADIAVKAAQTGHLVFSTLHTNRAAASLTRLLNMGVAPFHIAASVNLVVAQRLVRKLCPHCREVWERPSENVL
AAAGFTLQDLAQDWPLYRPKGCAACRGKGYRGRIGVFELMPVSQAMRRAMMEGAGELDLAALAAREGMVDLRRAGLLKVM
AGATTLAEVDAVVGR

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=940022 V6667_RS06800 WP_338809077.1 1470956..1472683(-) (pilF) [Neisseria leonii strain CCUG 45853]
ATGAATCGGGCAGTACGGCGGCAGGCCGTCTGTTTTGGATCGGCGGAGCCGGAAAACGGGTTGGCGGACGGAGGCGGTAT
GGCATCGGGGGCATTGCGGATTTTGGCCGGACATTTCGGTTGGGACGGAACGGTGTTGCGCCGTTGCGAAGAATGGGCGC
GGCAGGGGCTGCTGCTGCCCAAGGTGTTTGAAGAGGGTCTGTGTACGCCCGATGAGGCAGCGGCGGTGCTGGCGCAGGCG
GTCGGGCGGCCGTATGTGGATTTGGCGGCATTTTCATTTGCGTCCGAAGATACGGCGATGACGGCGGAGCAGATGCGCCG
TTTCGGCTGTGTGCCGCTGGCACGGCGGCAGGGGCGGCTGCTGGCGGGTGTGGCCGATCCGACGGATCCCGCGTTGCAGC
GGTTGGCTTTTGTCTGCGCCGAACCGGTCGATCTGGCGGTGGTGCGCTACGATCTGCTGGTGCAGCGGCTGGCAGAGTCG
GGTGGCGGTACGGCGGGGATTCTGGCGCAAATGCCGCAGGAGGAAGCGGGAACGGCCGCCGCTTGGGCGGAAGAAGCGGG
CGACGGGCCGGTGGCGCGTTTTGTGCAGCAGGTGTTGGAAGATGCGTGGGCGGCGGGTGCGTCGGACGTGCATTTCGAGT
TTTACGAGCAGGCGGCGCGGGTGCGTTTCCGCGTGGACGGTGTGCTGCGCGAAGTGGTGCAGCCGCCTCATGCGGTCAGA
CGGCGGCTGGTGTCGCGGATTAAAGTGATGGCGGGGCTGGATATTGCCGAACAGCGGCTGCCGCAGGACGGGCGTTTGCA
GACGGTTTTGGGCGGGCGCACGGCGGATTTGCGCATCAGCACGCTGCCGACGCTTTTCGGCGAAAAAGTGGTGGTGCGGA
TTCTGAACGGCGATGCGCAGGCGCCGGATTTGGCGCGTTTGGGGCTGGAGCCGTGCCAATATGACTTGCTGCTGGCCGAA
ATCCGCCGCCCTTACGGCATGATTCTGGTAACCGGGCCGACCGGTTCGGGCAAAACGCTGTCGCTGTATGCCTGTCTGAA
CGAATTGAACAGCGAGCGGGTGAATATTGCGGCGGTGGAAGACCCGGCGGAAATCAATTTGGCGGGCATCAATCAGGTGA
ATGTGAACGAGAAGCAGGGGCTGACGTTTGCCGAGGCTCTGCGGGCGTTTCTGCGGCAGGATCCCGATGTGCTGATGGTG
GGCGAAATCCGCGATTTGGAAACGGCCGATATTGCTGTCAAGGCGGCGCAGACGGGGCATTTGGTGTTTTCCACGCTGCA
TACCAACCGCGCGGCGGCCAGTCTGACGCGGCTGCTGAATATGGGGGTGGCGCCGTTCCATATTGCCGCTTCGGTGAATC
TGGTGGTGGCGCAGCGGCTGGTACGTAAGCTGTGTCCGCATTGCCGCGAAGTGTGGGAGCGGCCGTCTGAAAACGTGTTG
GCGGCGGCAGGGTTTACGCTGCAGGATTTGGCGCAGGATTGGCCGCTGTACCGTCCCAAAGGCTGCGCGGCCTGCCGAGG
TAAGGGTTATCGGGGGCGTATCGGCGTATTCGAGCTGATGCCGGTCAGTCAGGCAATGCGGCGGGCGATGATGGAGGGAG
CGGGCGAATTGGATTTGGCGGCATTGGCCGCACGGGAGGGTATGGTGGATTTGCGCCGTGCCGGATTGCTGAAAGTGATG
GCGGGTGCGACCACGCTGGCGGAAGTCGATGCGGTGGTGGGAAGGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-155)

Type II/IV secretion system protein (T2SSE)

(197-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.399

96.87

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

44.199

94.435

0.417
  pilB Vibrio cholerae strain A1552

45.283

92.174

0.417
  pilB Vibrio campbellii strain DS40M4

43.831

94.435

0.414
  pilB Acinetobacter baumannii D1279779

44.403

93.217

0.414
  pilB Acinetobacter baylyi ADP1

44.34

92.174

0.409
  pilB Legionella pneumophila strain ERS1305867

43.738

93.043

0.407