Detailed information NTG0938652

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VP418_RS14225
• Genome accession: NZ_CP145430
• Coordinates: 2947158..2948216 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain A20AB02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2942158..2953216

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP418_RS14200 (VP418_14200)	aroK	2942428..2942997 (-)	570	WP_000643712.1	shikimate kinase AroK	-
VP418_RS14205 (VP418_14205)	pilQ	2943009..2945174 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
VP418_RS14210 (VP418_14210)	pilP	2945242..2945769 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
VP418_RS14215 (VP418_14215)	pilO	2945780..2946520 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
VP418_RS14220 (VP418_14220)	pilN	2946517..2947158 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
VP418_RS14225 (VP418_14225)	pilM	2947158..2948216 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
VP418_RS14230 (VP418_14230)	ponA	2948379..2950934 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
VP418_RS14235 (VP418_14235)	-	2950952..2951776 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
VP418_RS14240 (VP418_14240)	-	2951779..2952111 (-)	333	WP_000169594.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=938652 VP418_RS14225 WP_002014066.1 2947158..2948216(-) (pilM) [Acinetobacter baumannii strain A20AB02]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=938652 VP418_RS14225 WP_002014066.1 2947158..2948216(-) (pilM) [Acinetobacter baumannii strain A20AB02]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423