Detailed information NTG0934863

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: V4Y05_RS03305
• Genome accession: NZ_CP144767
• Coordinates: 730100..731152 (+)
• Length: 350 a.a.
• NCBI ID: WP_053259356.1
• Uniprot ID: -
• Organism: Pseudomonas chlororaphis subsp. chlororaphis strain ATCC 9446
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 725100..736152

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4Y05_RS03295 (V4Y05_03295)	-	726052..727320 (+)	1269	WP_007925851.1	malic enzyme-like NAD(P)-binding protein	-
V4Y05_RS03300 (V4Y05_03300)	-	727448..729883 (-)	2436	WP_164523775.1	penicillin-binding protein 1A	-
V4Y05_RS03305 (V4Y05_03305)	pilM	730100..731152 (+)	1053	WP_053259356.1	pilus assembly protein PilM	Machinery gene
V4Y05_RS03310 (V4Y05_03310)	-	731152..731718 (+)	567	WP_053259357.1	PilN domain-containing protein	-
V4Y05_RS03315 (V4Y05_03315)	pilO	731715..732332 (+)	618	WP_053259358.1	type 4a pilus biogenesis protein PilO	-
V4Y05_RS03320 (V4Y05_03320)	-	732329..732856 (+)	528	WP_053259359.1	pilus assembly protein PilP	-
V4Y05_RS03325 (V4Y05_03325)	pilQ	732870..734942 (+)	2073	WP_053259360.1	type IV pilus secretin PilQ	Machinery gene
V4Y05_RS03330 (V4Y05_03330)	aroK	734947..735465 (+)	519	WP_009041824.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 350 a.a.
• Molecular weight: 37094.50 Da
• Isoelectric Point: 4.9018

>NTDB_id=934863 V4Y05_RS03305 WP_053259356.1 730100..731152(+) (pilM) [Pseudomonas chlororaphis subsp. chlororaphis strain ATCC 9446]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSPAARTVALVDIGATLTTLSVVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVLRSLQFFQASAPQHQVDSILLAGGSASVPGLVPLIDQRLGLPALIANPFADM
SIGAKVDAAALASEAPSLMIACGLALRSFD

Nucleotide

• Length: 1053 bp

>NTDB_id=934863 V4Y05_RS03305 WP_053259356.1 730100..731152(+) (pilM) [Pseudomonas chlororaphis subsp. chlororaphis strain ATCC 9446]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGTTTTCGGGTCGAGGCCTATGGCGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TAGTCGAGCCTGAAGGGGTCGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTATCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCTC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAAGTTCGCGAGGCGGCGCTCGAGCTC
GCCGGGCTGACGGCCCGAGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGGTCGTTCGCCCTGGCGGCCGGCTCGCCGGC
TGCCCGGACCGTGGCGCTGGTCGATATCGGCGCCACCCTGACCACCCTGAGCGTCGTGCATGAAGGGCAGATCGTCTATT
CCCGTGAGCAGTTGTTCGGCGGGCACCAACTGACCGAACACATCCAGCGTCGCTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCGAAAAAGCAGGGTGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCTTTTCAGGAGGCGGTTGTGCAACA
GGTTTTGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACACCAGGTCGATTCCATCCTGTTAGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGATCAGCGCCTGGGCTTGCCTGCGCTGATCGCCAACCCCTTCGCTGACATG
AGCATCGGCGCCAAGGTCGACGCCGCAGCCCTGGCCAGCGAAGCCCCGTCCTTGATGATCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-349)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.114	100	0.554
comM	Acinetobacter nosocomialis M2	54.83	100	0.551
comM	Acinetobacter baylyi ADP1	51.989	100	0.523
pilM	Legionella pneumophila strain ERS1305867	45.48	100	0.46