Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NUH87_RS02225 Genome accession   NZ_CP144470
Coordinates   567766..568935 (+) Length   389 a.a.
NCBI ID   WP_338524517.1    Uniprot ID   -
Organism   Pseudomonas batumici strain Koz11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 562766..573935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUH87_RS02205 (NUH87_02205) - 563486..564703 (+) 1218 WP_338524514.1 amino acid deaminase -
  NUH87_RS02210 (NUH87_02210) - 564715..565632 (+) 918 WP_338524515.1 sugar kinase -
  NUH87_RS02215 (NUH87_02215) - 565651..565926 (-) 276 WP_007932676.1 peptidylprolyl isomerase -
  NUH87_RS02220 (NUH87_02220) - 565984..567630 (-) 1647 WP_338524516.1 ATP-binding protein -
  NUH87_RS02225 (NUH87_02225) pilU 567766..568935 (+) 1170 WP_338524517.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NUH87_RS02230 (NUH87_02230) - 568963..569898 (-) 936 WP_338524518.1 carbohydrate kinase -
  NUH87_RS02235 (NUH87_02235) xylB 569905..571394 (-) 1490 Protein_446 xylulokinase -
  NUH87_RS02240 (NUH87_02240) - 571391..572860 (-) 1470 WP_338524520.1 mannitol dehydrogenase family protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43216.58 Da        Isoelectric Point: 6.7327

>NTDB_id=933977 NUH87_RS02225 WP_338524517.1 567766..568935(+) (pilU) [Pseudomonas batumici strain Koz11]
MDIHALLKTLAAQDGSDLYLSTGAPPCAKFNGVLKPLGSESLKLGEVAAIADGIMDPEQRLQFERELEMNLALSLPGVGR
FRINIFKQRNEVSIVARNIKLEIPRFEDLKLPPILLETIMEKRGLVLFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVNTQDGKRRAAVEVMLGTPTVRDFIKRNEFSELKGIMEKSEIIGMRTFDTAL
FELVVEGAIDEEEALKNADSVNNLRLRLKLHQDTAALTVQKTPPKPPIAAKELDAKDWGLVEDGNSPHG

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=933977 NUH87_RS02225 WP_338524517.1 567766..568935(+) (pilU) [Pseudomonas batumici strain Koz11]
ATGGATATCCACGCGTTGCTGAAAACCCTGGCCGCCCAGGACGGCTCGGACCTGTACCTGTCCACCGGTGCGCCGCCCTG
TGCCAAGTTCAATGGCGTGCTCAAGCCGTTGGGCAGCGAATCGCTGAAGCTCGGCGAGGTGGCGGCCATTGCCGACGGTA
TCATGGACCCCGAACAACGCCTGCAGTTCGAACGGGAACTGGAAATGAACCTGGCATTGTCCTTGCCCGGTGTCGGGCGG
TTCCGTATCAATATCTTCAAGCAGCGCAACGAAGTGTCCATCGTCGCGCGCAACATCAAGCTGGAAATCCCCCGTTTCGA
AGACCTCAAGCTGCCACCGATCCTGCTCGAGACCATCATGGAGAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACTGGTT
CAGGCAAGTCCACTTCGCTGGCGGCGCTGATCGACTACCGCAATCGCCACAGCAGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAGTCGATCATCAACCAGCGCGAAGTCGGGGTCGACACCCGCAGTTTCCACGC
GGCGCTGAAAAACACCCTGCGCCAGGCCCCGGATGTGATCCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCGTTCGCCGATACCGGGCACCTGGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTTTTCCCCGAAGACCGCCGGCCGCAACTGCTCAACGACCTGGGCAACAACCTGAAGGCCTTCGTCTCGCAGCG
CCTGGTCAATACCCAGGACGGCAAGCGCCGGGCGGCCGTGGAGGTGATGCTGGGTACGCCGACGGTGCGTGATTTCATCA
AGCGCAATGAGTTTTCCGAACTCAAGGGGATCATGGAGAAGTCCGAGATCATCGGCATGCGCACCTTCGACACGGCCTTG
TTCGAACTGGTGGTGGAAGGGGCGATCGACGAGGAGGAGGCGCTGAAGAACGCCGACTCGGTGAACAACCTGCGCCTGCG
TCTGAAGCTGCATCAGGATACCGCGGCCCTGACCGTGCAGAAGACCCCTCCCAAGCCGCCGATCGCGGCGAAGGAGTTGG
ATGCCAAGGATTGGGGCTTGGTGGAGGACGGCAACTCGCCGCACGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.056

91.26

0.512
  pilU Acinetobacter baylyi ADP1

52.958

91.26

0.483
  pilU Vibrio cholerae strain A1552

50.286

89.974

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.814

95.373

0.37