Detailed information NTG0933436

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V3Z63_RS02335
• Genome accession: NZ_CP144289
• Coordinates: 514365..515513 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CH1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 509365..520513

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3Z63_RS02310 (V3Z63_02310)	-	510516..510812 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
V3Z63_RS02315 (V3Z63_02315)	-	510809..511402 (-)	594	WP_031693619.1	YggT family protein	-
V3Z63_RS02320 (V3Z63_02320)	proC	511413..512234 (-)	822	WP_031693621.1	pyrroline-5-carboxylate reductase	-
V3Z63_RS02325 (V3Z63_02325)	-	512246..512938 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
V3Z63_RS02330 (V3Z63_02330)	pilT	513153..514187 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
V3Z63_RS02335 (V3Z63_02335)	pilU	514365..515513 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
V3Z63_RS02340 (V3Z63_02340)	czcD	515521..516420 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
V3Z63_RS02345 (V3Z63_02345)	-	516574..516978 (+)	405	WP_003084560.1	NINE protein	-
V3Z63_RS02350 (V3Z63_02350)	-	517221..518594 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
V3Z63_RS02355 (V3Z63_02355)	-	518591..519775 (+)	1185	WP_031693622.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=933436 V3Z63_RS02335 WP_003100959.1 514365..515513(+) (pilU) [Pseudomonas aeruginosa strain CH1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=933436 V3Z63_RS02335 WP_003100959.1 514365..515513(+) (pilU) [Pseudomonas aeruginosa strain CH1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGAGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382